PMID- 28626671
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2215-017X (Print)
IS  - 2215-017X (Electronic)
IS  - 2215-017X (Linking)
VI  - 4
DP  - 2014 Dec
TI  - Density-dependent enhancement of methane oxidation activity and growth of 
      Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp.
PG  - 128-133
LID - 10.1016/j.btre.2014.09.007 [doi]
AB  - Methanotrophs are a biological resource as they degrade the greenhouse gas 
      methane and various organic contaminants. Several non-methanotrophic bacteria 
      have shown potential to stimulate growth of methanotrophs when co-cultured, and 
      however, the ecology is largely unknown. Effects of Sphingopyxis sp. NM1 on 
      methanotrophic activity and growth of Methylocystis sp. M6 were investigated in 
      this study. M6 and NM1 were mixed at mixing ratios of 9:1, 1:1, and 1:9 (v/v), 
      using cell suspensions of 7.5 x 10(11) cells L(-1). Methane oxidation of M6 was 
      monitored, and M6 population was estimated using fluorescence in situ 
      hybridization (FISH). Real-time PCR was applied to quantify rRNA and expression 
      of transcripts for three enzymes involved in the methane oxidation pathway. NM1 
      had a positive effect on M6 growth at a 1:9 ratio (p < 0.05), while no 
      significant effects were observed at 9:1 and 1:1 ratios. NM1 enhanced the methane 
      oxidation 1.34-fold at the 1:9 ratio. NM1 increased the population density and 
      relative rRNA level of M6 by 2.4-fold and 5.4-fold at the 1:9 ratio, indicating 
      that NM1 stimulated the population growth of M6. NM1 increased the relative 
      transcriptional expression of all mRNA targets only at the 1:9 ratio. These 
      results demonstrated that NM1 enhanced the methanotrophic activity and growth of 
      M6, which was dependent on the proportion of NM1 present in the culture. This 
      stimulation can be used as management and enhancement strategies for 
      methanotrophic biotechnological processes.
FAU - Jeong, So-Yeon
AU  - Jeong SY
AD  - Global Top 5 Program, Department of Environmental Science and Engineering, Ewha 
      Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of 
      Korea.
FAU - Cho, Kyung-Suk
AU  - Cho KS
AD  - Global Top 5 Program, Department of Environmental Science and Engineering, Ewha 
      Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of 
      Korea.
FAU - Kim, Tae Gwan
AU  - Kim TG
AD  - Global Top 5 Program, Department of Environmental Science and Engineering, Ewha 
      Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of 
      Korea.
AD  - Department of ​Ecosystem Function, Bureau of Basic Ecological Research, National 
      Institute of Ecology, 1210, Geumgang-ro, Maseo-myeon, Seocheon-gun, 
      Chungcheongnam-do 325-813, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20140926
PL  - Netherlands
TA  - Biotechnol Rep (Amst)
JT  - Biotechnology reports (Amsterdam, Netherlands)
JID - 101637426
PMC - PMC5466132
OTO - NOTNLM
OT  - Methanotrophic activity
OT  - Methylocystis
OT  - Microbial interaction
OT  - Population growth
OT  - Sphingopyxis
EDAT- 2014/09/26 00:00
MHDA- 2014/09/26 00:01
PMCR- 2014/09/26
CRDT- 2017/06/20 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2014/09/11 00:00 [revised]
PHST- 2014/09/23 00:00 [accepted]
PHST- 2017/06/20 06:00 [entrez]
PHST- 2014/09/26 00:00 [pubmed]
PHST- 2014/09/26 00:01 [medline]
PHST- 2014/09/26 00:00 [pmc-release]
AID - S2215-017X(14)00041-1 [pii]
AID - 10.1016/j.btre.2014.09.007 [doi]
PST - epublish
SO  - Biotechnol Rep (Amst). 2014 Sep 26;4:128-133. doi: 10.1016/j.btre.2014.09.007. 
      eCollection 2014 Dec.