PMID- 28629357 OWN - NLM STAT- MEDLINE DCOM- 20170821 LR - 20211204 IS - 1472-6882 (Electronic) IS - 1472-6882 (Linking) VI - 17 IP - 1 DP - 2017 Jun 19 TI - XuefuZhuyu decoction protected cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy. PG - 325 LID - 10.1186/s12906-017-1822-0 [doi] LID - 325 AB - BACKGROUND: XuefuZhuyu decoction (XFZY) is a well-known traditional Chinese herbal medicine for the treatment of various cardiovascular diseases, such as unstable angina pectoris and myocardial ischemia-reperfusion injury. However, the mechanism by which XFZY contributes to the amelioration of cardiac injury remains unclear. METHODS: H9C2 cells were cultured under the hypoxic condition for 10 h and reoxygenated for 2 h. In the presence of various concentrations of XFZY for 12 h, the cell viability was measured by MTT assay. The protective effect of XFZY in hypoxia/reoxygenation (H/R) cell model was confirmed by measuring the amount of LDH released into the extracellular fluid. Cell apoptosis was measured by western blotting. The autophagy level of H9C2 cells and the correlative pathway were determined by transmission electron microscopy, Cyto-ID(R) Autophagy Detection Kit, and western blotting. RESULTS: In this study, we investigated the effects of XFZY on H/R induced cardiac injury. The results showed that treatment with XFZY significantly inhibited autophagy induced by H/R, with decreased formation of autophagosomes as well as the expression of LC3-II/LC3-I ratio and Beclin 1 after H/R. Importantly, inhibition of autophagy by XFZY resulted in enhanced cell viability and decreased apoptosis. XFZY also inhibited the activation of AMPK and upregulated the phosphorylation of mammalian target of Rapamycin (mTOR). CONCLUSIONS: The cardioprotective effects of XFZY during H/R were mediated by inhibiting autophagy via regulating AMPK-mTOR signaling pathways. FAU - Shi, Xiaowen AU - Shi X AD - Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China. FAU - Zhu, Haiyan AU - Zhu H AD - Department of Microbiological & Biochemical Pharmacy, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, People's Republic of China. haiyanzhu@fudan.edu.cn. FAU - Zhang, Yuanyuan AU - Zhang Y AD - Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, People's Republic of China. FAU - Zhou, Mingmei AU - Zhou M AD - Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China. FAU - Tang, Danli AU - Tang D AD - Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, No.16, Nanxiao Road, Dongzhimen, Beijing, 100700, People's Republic of China. FAU - Zhang, Huamin AU - Zhang H AD - Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, No.16, Nanxiao Road, Dongzhimen, Beijing, 100700, People's Republic of China. zhanghm@mail.cintcm.ac.cn. LA - eng PT - Journal Article DEP - 20170619 PL - England TA - BMC Complement Altern Med JT - BMC complementary and alternative medicine JID - 101088661 RN - 0 (Drugs, Chinese Herbal) RN - EC 2.7.1.1 (mTOR protein, rat) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) SB - IM MH - AMP-Activated Protein Kinases/genetics/metabolism MH - Animals MH - Apoptosis/drug effects MH - Autophagy/*drug effects MH - Cell Line MH - Cell Survival/drug effects MH - Drugs, Chinese Herbal/*pharmacology MH - Humans MH - Hypoxia/*complications/surgery MH - Male MH - Myocardial Reperfusion Injury/*drug therapy/etiology/metabolism/physiopathology MH - Myocytes, Cardiac/cytology/*drug effects/metabolism MH - Phosphorylation/drug effects MH - Rats MH - Rats, Sprague-Dawley MH - TOR Serine-Threonine Kinases/genetics/metabolism PMC - PMC5477241 OTO - NOTNLM OT - Autophagy OT - Hypoxia/reoxygenation OT - Ischemic/reperfusion OT - XuefuZhuyu decoction OT - mTOR EDAT- 2017/06/21 06:00 MHDA- 2017/08/22 06:00 PMCR- 2017/06/19 CRDT- 2017/06/21 06:00 PHST- 2017/02/16 00:00 [received] PHST- 2017/06/05 00:00 [accepted] PHST- 2017/06/21 06:00 [entrez] PHST- 2017/06/21 06:00 [pubmed] PHST- 2017/08/22 06:00 [medline] PHST- 2017/06/19 00:00 [pmc-release] AID - 10.1186/s12906-017-1822-0 [pii] AID - 1822 [pii] AID - 10.1186/s12906-017-1822-0 [doi] PST - epublish SO - BMC Complement Altern Med. 2017 Jun 19;17(1):325. doi: 10.1186/s12906-017-1822-0.