PMID- 28633489
OWN - NLM
STAT- MEDLINE
DCOM- 20180510
LR  - 20180510
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 97
IP  - 1
DP  - 2017 Jul 1
TI  - Effects of early pregnancy on uterine lymphocytes and endometrial expression of 
      immune-regulatory molecules in dairy heifers.
PG  - 104-118
LID - 10.1093/biolre/iox061 [doi]
AB  - Natural killer (NK) cells are essential for establishment of human and rodent 
      pregnancies. The function of these and other cytotoxic T cells (CTL) is 
      controlled by stimulatory and inhibitory signaling. A role for cytotoxic cells 
      during early pregnancy in cattle has not been described, but regulation of their 
      function at the fetal-maternal interface is thought to be critical for conceptus 
      survival. The hypothesis that the relative abundance of CTL and expression of 
      inhibitory signaling molecules is increased by the conceptus during early 
      pregnancy was tested. The proportions of lymphoid lineage cells and expression of 
      inhibitory signaling molecules in the endometrium during early pregnancy in dairy 
      heifers were determined using flow cytometry, immunofluorescence, and real-time 
      PCR on days 17 and 20 of pregnancy and day 17 of the estrous cycle. Results 
      revealed an increased percentage of NKp46+ and CD8+ cells in the uterus of 
      pregnant heifers. Furthermore, a large percentage of uterine immune cells 
      coexpressed these proteins. Compared to cyclic heifers, CD45+ uterine cells from 
      pregnant heifers exhibited greater degranulation. Endometrium from pregnant 
      heifers had greater mRNA abundance for the inhibitory molecules, CD274 and 
      lymphocyte activating gene 3 (LAG3), and greater cytotoxic T 
      lymphocyte-associated protein 4 (CTLA4), molecules that can interact with 
      receptors on antigen-presenting cells and induce lymphocyte tolerance. This study 
      demonstrates a dynamic regulation of both cytotoxic immune cells and tolerogenic 
      molecules during the peri-implantation period that may be required to support 
      establishment of pregnancy and placentation.
CI  - (c) The Authors 2017. Published by Oxford University Press on behalf of Society for 
      the Study of Reproduction. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Vasudevan, Sreelakshmi
AU  - Vasudevan S
AD  - Department of Animal Science, Center for Reproductive Biology and Health, Huck 
      Institutes of the Life Sciences, Pennsylvania State University, University Park, 
      Pennsylvania, USA.
AD  - VA Medical Center, San Francisco, California, USA.
FAU - Kamat, Manasi M
AU  - Kamat MM
AD  - Department of Animal Science, Center for Reproductive Biology and Health, Huck 
      Institutes of the Life Sciences, Pennsylvania State University, University Park, 
      Pennsylvania, USA.
AD  - City of Hope, Duarte, California, USA.
FAU - Walusimbi, Sadhat S
AU  - Walusimbi SS
AD  - Department of Animal Science, Center for Reproductive Biology and Health, Huck 
      Institutes of the Life Sciences, Pennsylvania State University, University Park, 
      Pennsylvania, USA.
AD  - Department of Agricultural Production, School of Agricultural Sciences, College 
      of Agricultural and Environmental Sciences, Makerere University, Kampala, Uganda.
FAU - Pate, Joy L
AU  - Pate JL
AD  - Department of Animal Science, Center for Reproductive Biology and Health, Huck 
      Institutes of the Life Sciences, Pennsylvania State University, University Park, 
      Pennsylvania, USA.
FAU - Ott, Troy L
AU  - Ott TL
AD  - Department of Animal Science, Center for Reproductive Biology and Health, Huck 
      Institutes of the Life Sciences, Pennsylvania State University, University Park, 
      Pennsylvania, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Female
MH  - Gene Expression Regulation/*immunology
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-10/genetics/metabolism
MH  - Killer Cells, Natural
MH  - Lymphocytes/*physiology
MH  - Pregnancy
MH  - *Pregnancy, Animal/physiology
MH  - Uterus/*cytology/metabolism
OTO - NOTNLM
OT  - cattle
OT  - cytotoxic T cells
OT  - endometrium
OT  - natural killer cells
OT  - pregnancy
EDAT- 2017/06/22 06:00
MHDA- 2018/05/11 06:00
CRDT- 2017/06/22 06:00
PHST- 2017/05/30 00:00 [received]
PHST- 2017/06/15 00:00 [accepted]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2018/05/11 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - 3869920 [pii]
AID - 10.1093/biolre/iox061 [doi]
PST - ppublish
SO  - Biol Reprod. 2017 Jul 1;97(1):104-118. doi: 10.1093/biolre/iox061.