PMID- 28634074
OWN - NLM
STAT- MEDLINE
DCOM- 20170811
LR  - 20171129
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 490
IP  - 3
DP  - 2017 Aug 26
TI  - Distinct regulation of activity-dependent transcription of immediate early genes 
      in cultured rat cortical neurons.
PG  - 682-687
LID - S0006-291X(17)31232-9 [pii]
LID - 10.1016/j.bbrc.2017.06.101 [doi]
AB  - The activity-regulated expression of immediate early genes (IEGs) contributes to 
      long-lasting neuronal functions underlying long-term memory. However, their 
      response properties following neuronal activity are unique and remain poorly 
      understood. To address this knowledge gap, here we further investigated the 
      response properties of two representative IEGs, c-fos and brain-derived 
      neurotrophic factor (Bdnf). Treatment of cultured cortical cells with KCl 
      produces a depolarization process that results in the increase of intracellular 
      calcium concentration in a KCl concentration-dependent manner. Consistent with 
      this increase, c-fos expression was induced in a KCl concentration-dependent 
      manner. In contrast, however, Bdnf expression was optimally activated by both 25 
      and 50 mM concentration of KCl. Similar results were observed when the cells were 
      treated with okadaic acid, which inhibits protein phosphatases and elicits the 
      hyper-phosphorylation of signaling molecules. Thus, Bdnf expression is strictly 
      regulated by a neuronal activity threshold in an all or nothing manner, whereas 
      c-fos expression is activated in a neuronal activity-dependent manner. Our 
      findings also suggest that these differential responses might be due to the 
      presence or absence of a TATA box.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Fukuchi, Mamoru
AU  - Fukuchi M
AD  - Laboratory of Molecular Neuroscience, Faculty of Pharmacy, Takasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma 370-0033, Japan; 
      Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan. Electronic address: fukuchi@takasaki-u.ac.jp.
FAU - Sanabe, Tomofumi
AU  - Sanabe T
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan.
FAU - Watanabe, Toshifumi
AU  - Watanabe T
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan.
FAU - Kubota, Takane
AU  - Kubota T
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan.
FAU - Tabuchi, Akiko
AU  - Tabuchi A
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan.
FAU - Tsuda, Masaaki
AU  - Tsuda M
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 
      930-0194, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170617
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cells, Cultured
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - *Genes, Immediate-Early
MH  - Memory, Long-Term
MH  - Neurons/cytology/*metabolism
MH  - Okadaic Acid/metabolism
MH  - Phosphorylation
MH  - Potassium Chloride/metabolism
MH  - Proto-Oncogene Proteins c-fos/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Transcriptional Activation
OTO - NOTNLM
OT  - BDNF
OT  - IEG
OT  - Neuronal activity
OT  - Okadaic acid
OT  - Transcription
OT  - c-fos
EDAT- 2017/06/22 06:00
MHDA- 2017/08/12 06:00
CRDT- 2017/06/22 06:00
PHST- 2017/05/31 00:00 [received]
PHST- 2017/06/17 00:00 [accepted]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2017/08/12 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - S0006-291X(17)31232-9 [pii]
AID - 10.1016/j.bbrc.2017.06.101 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Aug 26;490(3):682-687. doi: 
      10.1016/j.bbrc.2017.06.101. Epub 2017 Jun 17.