PMID- 28634474
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Different Dynamic Distribution in Chickens and Ducks of the Hypervirulent, Novel 
      Genotype Fowl Adenovirus Serotype 4 Recently Emerged in China.
PG  - 1005
LID - 10.3389/fmicb.2017.01005 [doi]
LID - 1005
AB  - A hypervirulent fowl adenovirus serotype 4 (FAdV-4) has caused 
      hepatitis-hydropericardium syndrome (HHS) with mortalities that range from 30 to 
      80% in outbreaks across China since 2015. The FAdV-4 strain was characterized as 
      a novel genotype based on the specific genome characteristics. However, our 
      understanding of the dynamic distribution, tissue tropism, and pathogenesis of 
      the novel FAdV-4 is incomplete. In this study, a new, sensitive and 
      FAdV-4-specific real-time PCR was developed and applied to detect the dynamic 
      distribution of the duck origin, novel FAdV-4 strain HLJDAd15 in experimentally 
      infected special-pathogen free (SPF) chickens and ducks. Notably, the 
      pathogenicity and replication pattern of HLJDAd15 were completely different 
      between chickens and ducks. Severe hydropericardium and 10% mortality were 
      induced in chickens, whereas no clinical signs were observed in any duck. The 
      virus replicated was detected throughout the study in both chickens and ducks. 
      However, only one replication peak with a high virus concentration appeared in 
      chickens at 5 days post infection (dpi), whereas two peaks with relatively low 
      virus titres appeared in ducks at 7 and 21 dpi. Thus, ducks could be a natural 
      reservoir of the novel FAdV-4 absent of clinical signs, and a new transmission 
      route from ducks shedding FAdV-4 continually to chickens was revealed, which 
      might aggravate the outbreak of HHS in chickens. This study provides the first 
      accurate quantitative data for the replication kinetics of the novel FAdV-4 in 
      different hosts. The different pathogenicity, dynamic distribution and 
      replication pattern in chickens and ducks provide a foundation for further 
      clarification of the pathogenesis of the novel FAdV-4.
FAU - Pan, Qing
AU  - Pan Q
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Yang, Yanchao
AU  - Yang Y
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Shi, Zhibin
AU  - Shi Z
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Liu, Linlin
AU  - Liu L
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Gao, Yulong
AU  - Gao Y
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Qi, Xiaole
AU  - Qi X
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Liu, Changjun
AU  - Liu C
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Zhang, Yanping
AU  - Zhang Y
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Cui, Hongyu
AU  - Cui H
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
FAU - Wang, Xiaomei
AU  - Wang X
AD  - Division of Aivan Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural SciencesHarbin, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Disease and ZoonosesYangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20170606
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5459905
OTO - NOTNLM
OT  - different hosts
OT  - duck origin
OT  - dynamic distribution
OT  - novel FAdV-4
OT  - real-time PCR
EDAT- 2017/06/22 06:00
MHDA- 2017/06/22 06:01
PMCR- 2017/06/06
CRDT- 2017/06/22 06:00
PHST- 2017/03/27 00:00 [received]
PHST- 2017/05/19 00:00 [accepted]
PHST- 2017/06/22 06:00 [entrez]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2017/06/22 06:01 [medline]
PHST- 2017/06/06 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.01005 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Jun 6;8:1005. doi: 10.3389/fmicb.2017.01005. eCollection 
      2017.