PMID- 28635380
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20171128
IS  - 1748-6963 (Electronic)
IS  - 1743-5889 (Linking)
VI  - 12
IP  - 14
DP  - 2017 Jul
TI  - Inorganic nanoparticles as potential regulators of immune response in dendritic 
      cells.
PG  - 1647-1660
LID - 10.2217/nnm-2017-0061 [doi]
AB  - AIM: The spontaneous adsorption of proteins on nanoparticles (NPs) in biological 
      media is exploited to prepare complexes of NPs and proteins from cancer cells' 
      lysates for application in cancer immunotherapy. MATERIALS & METHODS: Gold (Au) 
      and silica NPs were synthesized, incubated with cancer cells' lysates and 
      characterized. Dendritic cells (DCs) were challenged with protein-coated NPs, 
      their maturation, viability and morphology were evaluated and lymphocytes T 
      proliferation was determined. RESULTS: Silica and Au NPs bound different pools of 
      biomolecules from lysates, and are therefore promising selective carriers for 
      antigens. When incubated with immature DCs, NPs were efficiently endocytosed 
      without cytotoxicity. Finally, protein-coated AuNPs promoted DC maturation and 
      DC-mediated lymphocyte proliferation, at variance with lysate alone and 
      protein-coated silica NPs, that did not promote DCs maturation. CONCLUSION: These 
      results demonstrate that the spontaneous formation of protein coronas on NPs 
      represents a possible approach to fast, easy, cost-effective DCs stimulation.
FAU - Fogli, Silvia
AU  - Fogli S
AD  - Department of Chemistry 'Ugo Schiff,' University of Florence, and CSGI, via della 
      Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
FAU - Montis, Costanza
AU  - Montis C
AD  - Department of Chemistry 'Ugo Schiff,' University of Florence, and CSGI, via della 
      Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
FAU - Paccosi, Sara
AU  - Paccosi S
AD  - Department of Health Sciences, Clinical Pharmacology & Oncology Section, 
      University of Florence, Viale Pieraccini 6, 50139, FI, Italy.
FAU - Silvano, Angela
AU  - Silvano A
AD  - Department of Experimental & Clinical Medicine, University of Florence, via Ugo 
      Schiff 6, 50019 Sesto Fiorentino, FI, Italy.
FAU - Michelucci, Elena
AU  - Michelucci E
AD  - Mass Spectrometry Center (CISM), University of Florence, via Ugo Schiff 6, 50019 
      Sesto Fiorentino, FI, Italy.
FAU - Berti, Debora
AU  - Berti D
AD  - Department of Chemistry 'Ugo Schiff,' University of Florence, and CSGI, via della 
      Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
FAU - Bosi, Alberto
AU  - Bosi A
AD  - Department of Experimental & Clinical Medicine, University of Florence, via Ugo 
      Schiff 6, 50019 Sesto Fiorentino, FI, Italy.
FAU - Parenti, Astrid
AU  - Parenti A
AD  - Department of Health Sciences, Clinical Pharmacology & Oncology Section, 
      University of Florence, Viale Pieraccini 6, 50139, FI, Italy.
FAU - Romagnoli, Paolo
AU  - Romagnoli P
AD  - Department of Experimental & Clinical Medicine, University of Florence, via Ugo 
      Schiff 6, 50019 Sesto Fiorentino, FI, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170621
PL  - England
TA  - Nanomedicine (Lond)
JT  - Nanomedicine (London, England)
JID - 101278111
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Protein Corona)
RN  - 7440-57-5 (Gold)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Adsorption
MH  - Cell Line, Tumor
MH  - Coated Materials, Biocompatible/chemistry/therapeutic use
MH  - Dendritic Cells/*immunology
MH  - Gold/chemistry
MH  - Humans
MH  - Immunotherapy/*methods
MH  - Nanoparticles/chemistry/*therapeutic use
MH  - Neoplasms/immunology/*therapy
MH  - *Protein Corona/chemistry/immunology
MH  - Silicon Dioxide/chemistry
OTO - NOTNLM
OT  - cancer immunotherapy
OT  - dendritic cells
OT  - lysate
OT  - nanoparticles
OT  - protein corona
EDAT- 2017/06/22 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/06/22 06:00
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - 10.2217/nnm-2017-0061 [doi]
PST - ppublish
SO  - Nanomedicine (Lond). 2017 Jul;12(14):1647-1660. doi: 10.2217/nnm-2017-0061. Epub 
      2017 Jun 21.