PMID- 28636071
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR  - 20180501
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 55
IP  - 2
DP  - 2017
TI  - The role of homocysteine in seminal vesicles remodeling in rat.
PG  - 62-73
LID - 10.5603/FHC.a2017.0010 [doi]
AB  - INTRODUCTION: Elevated plasma homocysteine (Hcy) levels have been associated with 
      several tissue injuries including heart and liver fibrosis. In these diseases, 
      hyperhomocysteinemia (Hhcy) plays a major role in modulating the alteration of 
      the balance between matrix metalloproteinases (MMP) and tissue inhibitors of 
      metalloproteinases (TIMPs), leading to the pathological accumulation of 
      extracellular matrix (ECM) proteins. Since the effect of Hhcy on ECM of seminal 
      vesicle was not studied, the aim of our research was to check if Hcy can induce a 
      remodeling within seminal vesicles ECM. MATERIAL AND METHODS: The study was 
      conducted in 22 adult male Wistar rats. The rats were divided into two groups: a 
      control group, which received standard diet and tap water; the treated group 
      received the same diet and water supplemented with solution of L-methionine (200 
      mg/kg b.w./day) for 6 months. Plasma homocysteine concentration was measured. 
      Histological changes were observed with light microscope. The presence of 
      collagen I and III and metalloproteinases (2, 3, 7 and 9) in the seminal vesicles 
      was examined using immunohistochemistry and Western blotting. RESULTS: Plasma Hcy 
      levels increased significantly after methionine treatment and interfered 
      significantly with body weight in treated rats. The content of fibrillar 
      collagens (I and III) in the wall of seminal vesicles was elevated in 
      hyperhomocysteinemic rats. Moreover, we found that hyperhomocysteinemia increased 
      the expression of MMP-2, -3, -7 and -9 in seminal vesicles of experimental rats. 
      CONCLUSIONS: Increased plasma concentration of Hcy accompanied by the 
      accumulation of collagen and upregulation of MMPs in rat seminal vesicles might 
      contribute to the remodeling of seminal vesicles.
FAU - Ghoul, Adel
AU  - Ghoul A
AD  - Laboratory of Cellular and Molecular Biology (BCM), Extracellular Matrix, Faculty 
      of Biological Sciences (FSB), USTHB, El Alia, Algiers, Algeria, Algeria. 
      ghoul.adel@yahoo.fr.
FAU - Moudilou, Elara
AU  - Moudilou E
FAU - Cherifi, Mohamed El Hadi
AU  - Cherifi MEH
FAU - Zerrouk, Fouzia
AU  - Zerrouk F
FAU - Chaouad, Billel
AU  - Chaouad B
FAU - Moulahoum, Anissa
AU  - Moulahoum A
FAU - Aouichat-Bouguerra, Souhila
AU  - Aouichat-Bouguerra S
FAU - Othmani, Khira
AU  - Othmani K
FAU - Exbrayat, Jean-Marie
AU  - Exbrayat JM
FAU - Benazzoug, Yasmina
AU  - Benazzoug Y
LA  - eng
PT  - Journal Article
DEP - 20170621
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Body Weight
MH  - Homocysteine/blood/*metabolism
MH  - Male
MH  - Matrix Metalloproteinases/metabolism
MH  - Organ Size
MH  - Rats
MH  - Rats, Wistar
MH  - Seminal Vesicles/*metabolism
OTO - NOTNLM
OT  - IHC
OT  - Western blot
OT  - collagen I
OT  - collagen III
OT  - hyperhomocysteinemia
OT  - metalloproteinases
OT  - rat
OT  - seminal vesicles
EDAT- 2017/06/22 06:00
MHDA- 2018/05/02 06:00
CRDT- 2017/06/22 06:00
PHST- 2016/05/12 00:00 [received]
PHST- 2017/06/13 00:00 [accepted]
PHST- 2017/05/19 00:00 [revised]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - VM/OJS/J/47175 [pii]
AID - 10.5603/FHC.a2017.0010 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2017;55(2):62-73. doi: 10.5603/FHC.a2017.0010. Epub 
      2017 Jun 21.