PMID- 28636626
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 6
DP  - 2017
TI  - Digestible lysine requirements of male broilers from 1 to 42 days of age 
      reassessed.
PG  - e0179665
LID - 10.1371/journal.pone.0179665 [doi]
LID - e0179665
AB  - Three experiments were conducted separately to estimate the digestible Lys (dig. 
      Lys) requirements of Cobb x Cobb 500 male broilers using different statistical 
      models. For each experiment, 1,200 chicks were housed in 48 floor pens in a 
      completely randomized design with 6 treatments and 8 replicates. Broilers were 
      fed diets with increasing dig. Lys levels from 1 to 12 d (Exp. 1), from 12 to 28 
      d (Exp. 2), and 28 to 42 d (Exp. 3). Increasing dig. Lys levels were equally 
      spaced from 0.97 to 1.37% in Exp. 1, 0.77 to 1.17% in Exp. 2, and 0.68 to 1.07% 
      in Exp. 3. The lowest dig. Lys diets were not supplemented with L-Lysine and all 
      other essential AA met or exceeded recommendations. In Exp. 3, six birds per pen 
      were randomly selected from each replication to evaluate carcass and breast 
      yields. Digestible Lys requirements were estimated by quadratic polynomial (QP), 
      linear broken-line (LBL), quadratic broken-line (QBL), and exponential asymptotic 
      (EA) models. Overall, dig. Lys requirements varied among response variables and 
      statistical models. Increasing dietary dig. Lys had a positive effect on BW, 
      carcass and breast yields. Levels of dig. Lys that optimized performance using 
      QP, LBL, QBL, and EA models were 1.207, 1.036, 1.113, and 1.204% for BWG and 
      1.190, 1.027, 1.100, and 1.172% for FCR in Exp. 1; 1.019, 0.853, 0.944; 1.025% 
      for BWG and 1.050, 0.879, 1.032, and 1.167% for FCR in Exp. 2; and 0.960, 0.835, 
      0.933, and 1.077% for BWG, 0.981, 0.857, 0.963, and 1.146% for FCR in Exp. 3. The 
      QP, LBL, QBL, and EA also estimated dig. Lys requirements as 0.941, 0.846, 0.925, 
      and 1.070% for breast meat yield in Exp. 3. In conclusion, Lys requirements vary 
      greatly according to the statistical analysis utilized; therefore, the origin of 
      requirement estimation must be taken into account in order to allow adequate 
      comparisons between references.
FAU - Cemin, Henrique Scher
AU  - Cemin HS
AD  - Department of Animal Science, Federal University of Rio Grande do Sul, Porto 
      Alegre, Rio Grande do Sul, Brazil.
FAU - Vieira, Sergio Luiz
AU  - Vieira SL
AUID- ORCID: 0000-0001-6206-9183
AD  - Department of Animal Science, Federal University of Rio Grande do Sul, Porto 
      Alegre, Rio Grande do Sul, Brazil.
FAU - Stefanello, Catarina
AU  - Stefanello C
AD  - Department of Animal Science, Federal University of Rio Grande do Sul, Porto 
      Alegre, Rio Grande do Sul, Brazil.
FAU - Kipper, Marcos
AU  - Kipper M
AD  - Department of Animal Science, Federal University of Rio Grande do Sul, Porto 
      Alegre, Rio Grande do Sul, Brazil.
FAU - Kindlein, Liris
AU  - Kindlein L
AD  - Department of Preventive Veterinary Medicine, Federal University of Rio Grande do 
      Sul, Porto Alegre, Rio Grande do Sul, Brazil.
FAU - Helmbrecht, Ariane
AU  - Helmbrecht A
AD  - Evonik Nutrition & Care GmbH, Hanau-Wolfgang, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170621
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animal Feed
MH  - *Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Chickens/*growth & development/physiology
MH  - Female
MH  - Lysine/*metabolism
MH  - Male
MH  - Models, Biological
MH  - *Nutritional Requirements
MH  - Time Factors
PMC - PMC5479556
COIS- Competing Interests: The authors declare that the submitted work had financial 
      support from Evonik Nutrition & Care GmbH. Ariane Helmbrecht is employed by 
      Evonik Nutrition & Care GmbH. There are no other professional or personal 
      interests that might have influenced the performance or presentation of the work 
      described in this manuscript. This does not alter our adherence to PLOS ONE 
      policies on sharing data and materials.
EDAT- 2017/06/22 06:00
MHDA- 2017/09/16 06:00
PMCR- 2017/06/21
CRDT- 2017/06/22 06:00
PHST- 2017/03/13 00:00 [received]
PHST- 2017/06/01 00:00 [accepted]
PHST- 2017/06/22 06:00 [entrez]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
PHST- 2017/06/21 00:00 [pmc-release]
AID - PONE-D-17-09773 [pii]
AID - 10.1371/journal.pone.0179665 [doi]
PST - epublish
SO  - PLoS One. 2017 Jun 21;12(6):e0179665. doi: 10.1371/journal.pone.0179665. 
      eCollection 2017.