PMID- 28636823
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20190329
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 9
IP  - 27
DP  - 2017 Jul 12
TI  - How Bacteria Respond to Material Stiffness during Attachment: A Role of 
      Escherichia coli Flagellar Motility.
PG  - 22176-22184
LID - 10.1021/acsami.7b04757 [doi]
AB  - Material stiffness has been shown to have potent effects on bacterial attachment 
      and biofilm formation, but the mechanism is still unknown. In this study, 
      response to material stiffness by Escherichia coli during attachment was 
      investigated with biofilm assays and cell tracking using the Automated 
      Contour-base Tracking for in Vitro Environments (ACTIVE) computational algorithm. 
      By comparing the movement of E. coli cells attached on poly(dimethylsiloxane) 
      (PDMS) surfaces of different Young's moduli (0.1 and 2.6 MPa, prepared by 
      controlling the degree of cross-linking) using ACTIVE, attached cells on stiff 
      surfaces were found more motile during early stage biofilm formation than those 
      on soft surfaces. To investigate if motility is important to bacterial response 
      to material stiffness, we compared E. coli RP437 and its isogenic mutants of 
      flagellar motor (motB) and synthesis of flagella (fliC) and type I fimbriae 
      (fimA) for attachment on 0.1 and 2.6 MPa PDMS surfaces. The motB mutant exhibited 
      defects in response to PDMS stiffness (based on cell counting and tracking with 
      ACTIVE), which was recovered by complementing the motB gene. Unlike motB results, 
      mutants of fliC and fimA did not show significant defects on both face-up and 
      face-down surfaces. Collectively, these findings suggest that E. coli cells can 
      actively respond to material stiffness during biofilm formation, and motB is 
      involved in this response.
FAU - Song, Fangchao
AU  - Song F
AD  - Department of Biomedical and Chemical Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Syracuse Biomaterials Institute, Syracuse University , Syracuse, New York 13244, 
      United States.
FAU - Brasch, Megan E
AU  - Brasch ME
AD  - Department of Biomedical and Chemical Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Syracuse Biomaterials Institute, Syracuse University , Syracuse, New York 13244, 
      United States.
FAU - Wang, Hao
AU  - Wang H
AUID- ORCID: 0000-0002-5808-0256
AD  - Department of Biomedical and Chemical Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Syracuse Biomaterials Institute, Syracuse University , Syracuse, New York 13244, 
      United States.
FAU - Henderson, James H
AU  - Henderson JH
AD  - Department of Biomedical and Chemical Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Syracuse Biomaterials Institute, Syracuse University , Syracuse, New York 13244, 
      United States.
FAU - Sauer, Karin
AU  - Sauer K
AD  - Department of Biological Science, Binghamton University , Binghamton, New York 
      13902, United States.
FAU - Ren, Dacheng
AU  - Ren D
AUID- ORCID: 0000-0002-6517-906X
AD  - Department of Biomedical and Chemical Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Syracuse Biomaterials Institute, Syracuse University , Syracuse, New York 13244, 
      United States.
AD  - Department of Civil and Environmental Engineering, Syracuse University , 
      Syracuse, New York 13244, United States.
AD  - Department of Biology, Syracuse University , Syracuse, New York 13244, United 
      States.
LA  - eng
GR  - R21 EY025750/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20170630
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Bacterial Proteins
MH  - Biofilms
MH  - *Escherichia coli
MH  - Fimbriae, Bacterial
MH  - Flagella
OTO - NOTNLM
OT  - attachment
OT  - bacteria
OT  - material stiffness
OT  - mechanosensing
OT  - motility
EDAT- 2017/06/22 06:00
MHDA- 2018/12/18 06:00
CRDT- 2017/06/22 06:00
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - 10.1021/acsami.7b04757 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2017 Jul 12;9(27):22176-22184. doi: 
      10.1021/acsami.7b04757. Epub 2017 Jun 30.