PMID- 28639010
OWN - NLM
STAT- MEDLINE
DCOM- 20180313
LR  - 20180313
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 101
IP  - 16
DP  - 2017 Aug
TI  - Modulation of calcium carbonate precipitation by exopolysaccharide in Bacillus 
      sp. JH7.
PG  - 6551-6561
LID - 10.1007/s00253-017-8372-8 [doi]
AB  - Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion 
      supersaturation through its nucleation effect during the microbially induced 
      calcium carbonate precipitation (MICP) process. However, the supersaturation 
      effect of Ca(2+) via EPS in MICP has not been clearly demonstrated. Enhanced 
      exopolysaccharide production of the alkali- and halotolerant MICP-capable 
      bacteria, Bacillus sp. JH7, was achieved through glycerol addition. This was 
      demonstrated by measuring cellular precipitation and Congo red binding. 
      Interestingly, field emission scanning electron microscopy and energy-dispersive 
      X-ray spectrometry analysis demonstrated that there was no MICP under 
      glycerol-amended conditions. Although glycerol promoted exopolysaccharide capture 
      of Ca(2+) ions, Ca(2+) embedded onto EPS did not participate in MICP formation. 
      The pH was reduced in glycerol-added media, which led us to analyze high acetate 
      production under our test conditions. Purified glycerol-induced exopolysaccharide 
      showed a higher capacity of Ca(2+) capture than the control. Quantitative RT-PCR 
      analysis showed that three genes involved in exopolysaccharide production were 
      highly upregulated by glycerol. The amounts of three detected monosaccharides 
      (arabinose, glucose, and mannose) were altered by glycerol. Cell hydrophobicity 
      measurements indicated that glycerol could confer more hydrophilic 
      characteristics to cells, which might enhance Ca(2+) binding onto EPS. 
      Unexpectedly, our data demonstrated, for the first time, that glycerol could 
      promote exopolysaccharide and acetate production under our test condition, which 
      could inhibit MICP by reducing the availability of free Ca(2+).
FAU - Kim, Hyun Jung
AU  - Kim HJ
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea.
FAU - Shin, Bora
AU  - Shin B
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea.
FAU - Lee, Yun Suk
AU  - Lee YS
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea.
FAU - Park, Woojun
AU  - Park W
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea. wpark@korea.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20170622
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Acetates)
RN  - 0 (Monosaccharides)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (exopolysaccharide, Bacillus)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Acetates/metabolism
MH  - Bacillus/drug effects/genetics/*metabolism
MH  - Calcium Carbonate/chemistry/*metabolism
MH  - Chemical Precipitation
MH  - Glycerol/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microscopy, Electron, Scanning
MH  - Monosaccharides/analysis/metabolism
MH  - Polysaccharides, Bacterial/chemistry/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Spectrometry, X-Ray Emission
OTO - NOTNLM
OT  - Bacillus
OT  - Calcium carbonate
OT  - Exopolysaccharide
OT  - Glycerol
EDAT- 2017/06/24 06:00
MHDA- 2018/03/14 06:00
CRDT- 2017/06/23 06:00
PHST- 2017/02/08 00:00 [received]
PHST- 2017/05/25 00:00 [accepted]
PHST- 2017/05/24 00:00 [revised]
PHST- 2017/06/24 06:00 [pubmed]
PHST- 2018/03/14 06:00 [medline]
PHST- 2017/06/23 06:00 [entrez]
AID - 10.1007/s00253-017-8372-8 [pii]
AID - 10.1007/s00253-017-8372-8 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2017 Aug;101(16):6551-6561. doi: 
      10.1007/s00253-017-8372-8. Epub 2017 Jun 22.