PMID- 28639134
OWN - NLM
STAT- MEDLINE
DCOM- 20181224
LR  - 20181224
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 369
IP  - 3
DP  - 2017 Sep
TI  - KIFC1 and myosin Va: two motors for acrosomal biogenesis and nuclear shaping 
      during spermiogenesis of Portunus trituberculatus.
PG  - 625-640
LID - 10.1007/s00441-017-2638-4 [doi]
AB  - To investigate the molecular mechanisms underlying the spermiogenesis of the 
      swimming crab Portunus trituberculatus, full lengths of motor proteins KIFC1 and 
      myosin Va were cloned by rapid-amplification of cDNA ends from P. trituberculatus 
      testes cDNA, and their respective probes and specific antibodies were used to 
      track their localization during sperm maturation. Antisense probes were designed 
      from the gene sequences and used to detect the mRNA levels of each gene. 
      According to the results of fluorescence in situ hybridization (FISH), the 
      transcription of kifc1 and myosin Va began at the mid-stage of spermatids, with 
      the kifc1 mRNA being most active at the location where the acrosome cap was 
      formed and the myosin Va was more concentrated in the acrosome complex. 
      Immunofluorescence results showed that KIFC1 and myosin Va were highly expressed 
      in each stage of spermigenesis. In the early spermatids, they were randomly 
      dispersed in the cytoplasm together with cytoskeletons. At the mid-stage, the 
      motors were gathered above one side of the nucleus where the acrosome would later 
      form. In the late spermatids and mature sperm, the KIFC1 was closely distributed 
      in the perinuclear region, indicating its role in nucleus deformation. Myosin Va 
      was distributed in the acrosome complex until sperm maturity. This suggests 
      myosin Va's potential role in material transportation during acrosome formation 
      and maturation. The above results provide a preliminary illustration of the 
      essential roles of KIFC1 and myosin Va in the spermiogenesis of the swimming crab 
      P. trituberculatus.
FAU - Ma, Dan-Dan
AU  - Ma DD
AD  - The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, 
      Zhejiang, China.
FAU - Pan, Meng-Ying
AU  - Pan MY
AD  - The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, 
      Zhejiang, China.
FAU - Hou, Cong-Cong
AU  - Hou CC
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, People's 
      Republic of China.
FAU - Tan, Fu-Qing
AU  - Tan FQ
AD  - The First Affiliated Hospital, College of Medicine, Zhejiang University, 
      Hangzhou, 310003, China.
FAU - Yang, Wan-Xi
AU  - Yang WX
AD  - The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, 
      Zhejiang, China. wxyang@spermlab.org.
LA  - eng
GR  - LY15H040003/Zhejiang Provincial Natural Science Foundation of China/International
GR  - 41276151/National Natural Science Foundation of China/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170621
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Antibodies)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta Karyopherins)
RN  - EC 3.6.1.- (Myosin Type V)
SB  - IM
MH  - Acrosome/*metabolism
MH  - Actin Cytoskeleton/metabolism
MH  - Animals
MH  - Antibodies/metabolism
MH  - Brachyura/genetics/*metabolism
MH  - *Cell Nucleus Shape
MH  - Gene Expression Regulation
MH  - Male
MH  - Models, Biological
MH  - Myosin Type V/*metabolism
MH  - Phylogeny
MH  - Protein Domains
MH  - RNA, Messenger/genetics/metabolism
MH  - *Spermatogenesis/genetics
MH  - Time Factors
MH  - beta Karyopherins/chemistry/genetics/*metabolism
OTO - NOTNLM
OT  - Cytoskeleton
OT  - KIFC1
OT  - Myosin Va
OT  - Portunus Trituberculatus
OT  - Spermiogenesis
EDAT- 2017/06/24 06:00
MHDA- 2018/12/26 06:00
CRDT- 2017/06/23 06:00
PHST- 2016/12/27 00:00 [received]
PHST- 2017/05/02 00:00 [accepted]
PHST- 2017/06/24 06:00 [pubmed]
PHST- 2018/12/26 06:00 [medline]
PHST- 2017/06/23 06:00 [entrez]
AID - 10.1007/s00441-017-2638-4 [pii]
AID - 10.1007/s00441-017-2638-4 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2017 Sep;369(3):625-640. doi: 10.1007/s00441-017-2638-4. Epub 
      2017 Jun 21.