PMID- 28640008
OWN - NLM
STAT- MEDLINE
DCOM- 20180402
LR  - 20181113
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 28
IP  - 12
DP  - 2017 Aug 16
TI  - Brain-derived neurotrophic factor propeptide inhibits proliferation and induces 
      apoptosis in C6 glioma cells.
PG  - 726-730
LID - 10.1097/WNR.0000000000000828 [doi]
AB  - There are several forms of brain-derived neurotrophic factor (BDNF), the 
      precursor of BDNF, mature BDNF, and BDNF propeptide. They exert different effects 
      through different transmembrane receptor signaling systems. Precursor of BDNF is 
      enzymatically cleaved, either by intracellular or by extracellular proteases, to 
      generate mature BDNF and its propeptide (BDNF propeptide). The aim of this study 
      was to evaluate the potential molecular mechanisms that underlie the inhibition 
      of glioma cell growth by the BDNF propeptide. To achieve this, we examined the 
      expression of BDNF propeptide in C6 glioma cells. The 
      3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide assay and the 
      apoptosis assay were used to assess the effects of the BDNF propeptide on the 
      growth and apoptosis of glioma cells. We found that the BDNF propeptide promoted 
      C6 glioma cell apoptosis and decreased in-vitro cell growth. We also found using 
      western blot that cleaved caspase3 and B cell lymphoma 2 (Bcl2)-associated X 
      protein abundances increased, whereas Bcl2 abundance decreased. Our data suggest 
      that the BDNF propeptide may have an inhibitory effect on glioma through 
      activation of the caspase3 pathway.
FAU - Yang, Bangkun
AU  - Yang B
AD  - aDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 
      bDepartment of Neurosurgery cDepartment of Children's Medical Center dDepartment 
      of Orthopedics, Taihe Hospital, Hubei University of Medicine, Shiyan, People's 
      Republic of China.
FAU - Qin, Jun
AU  - Qin J
FAU - Nie, Ying
AU  - Nie Y
FAU - Li, Yong
AU  - Li Y
FAU - Chen, Qianxue
AU  - Chen Q
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Bax protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Brain/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*physiology
MH  - Cell Survival/physiology
MH  - Escherichia coli
MH  - Glioma/*metabolism
MH  - Humans
MH  - Mice
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Spinal Cord/metabolism
MH  - bcl-2-Associated X Protein/metabolism
PMC - PMC5502118
EDAT- 2017/06/24 06:00
MHDA- 2018/04/03 06:00
PMCR- 2017/07/09
CRDT- 2017/06/23 06:00
PHST- 2017/06/24 06:00 [pubmed]
PHST- 2018/04/03 06:00 [medline]
PHST- 2017/06/23 06:00 [entrez]
PHST- 2017/07/09 00:00 [pmc-release]
AID - 10.1097/WNR.0000000000000828 [doi]
PST - ppublish
SO  - Neuroreport. 2017 Aug 16;28(12):726-730. doi: 10.1097/WNR.0000000000000828.