PMID- 28640817
OWN - NLM
STAT- MEDLINE
DCOM- 20170920
LR  - 20210408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 6
DP  - 2017
TI  - An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red 
      fluorescent calcium indicator.
PG  - e0179460
LID - 10.1371/journal.pone.0179460 [doi]
LID - e0179460
AB  - Genetically encoded calcium indicators (GECIs) enable imaging of in vivo brain 
      cell activity with high sensitivity and specificity. In contrast to viral 
      infection or in utero electroporation, indicator expression in transgenic 
      reporter lines is induced noninvasively, reliably, and homogenously. Recently, 
      Cre/tTA-dependent reporter mice were introduced, which provide high-level 
      expression of green fluorescent GECIs in a cell-type-specific and inducible 
      manner when crossed with Cre and tTA driver mice. Here, we generated and 
      characterized the first red-shifted GECI reporter line of this type using 
      R-CaMP1.07, a red fluorescent indicator that is efficiently two-photon excited 
      above 1000 nm. By crossing the new R-CaMP1.07 reporter line to Cre lines driving 
      layer-specific expression in neocortex we demonstrate its high fidelity for 
      reporting action potential firing in vivo, long-term stability over months, and 
      versatile use for functional imaging of excitatory neurons across all cortical 
      layers, especially in the previously difficult to access layers 4 and 6.
FAU - Bethge, Philipp
AU  - Bethge P
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
FAU - Carta, Stefano
AU  - Carta S
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
FAU - Lorenzo, Dayra A
AU  - Lorenzo DA
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
FAU - Egolf, Ladan
AU  - Egolf L
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
FAU - Goniotaki, Despoina
AU  - Goniotaki D
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
AD  - Institute of Neuropathology, University Hospital of Zurich, Zurich, Switzerland.
FAU - Madisen, Linda
AU  - Madisen L
AD  - Allen Institute for Brain Science, Seattle, Washington, United States of America.
FAU - Voigt, Fabian F
AU  - Voigt FF
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
FAU - Chen, Jerry L
AU  - Chen JL
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
FAU - Schneider, Bernard
AU  - Schneider B
AD  - Brain Mind Institute, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, 
      Switzerland.
FAU - Ohkura, Masamichi
AU  - Ohkura M
AD  - Saitama University, Brain Science Institute, Saitama, Japan.
FAU - Nakai, Junichi
AU  - Nakai J
AD  - Saitama University, Brain Science Institute, Saitama, Japan.
FAU - Zeng, Hongkui
AU  - Zeng H
AD  - Allen Institute for Brain Science, Seattle, Washington, United States of America.
FAU - Aguzzi, Adriano
AU  - Aguzzi A
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
AD  - Institute of Neuropathology, University Hospital of Zurich, Zurich, Switzerland.
FAU - Helmchen, Fritjof
AU  - Helmchen F
AUID- ORCID: 0000-0002-8867-9569
AD  - Brain Research Institute, University of Zurich, Zurich, Switzerland.
AD  - Neuroscience Center Zurich (ZNZ), University of Zurich and ETH Zurich, Zurich, 
      Switzerland.
LA  - eng
GR  - R01 DA028298/DA/NIDA NIH HHS/United States
GR  - U01 NS090475/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20170622
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Gene Expression
MH  - Green Fluorescent Proteins/*genetics/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Imaging
MH  - Neocortex/diagnostic imaging/metabolism
MH  - *Photons
PMC - PMC5480891
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/06/24 06:00
MHDA- 2017/09/21 06:00
PMCR- 2017/06/22
CRDT- 2017/06/23 06:00
PHST- 2017/02/17 00:00 [received]
PHST- 2017/05/29 00:00 [accepted]
PHST- 2017/06/23 06:00 [entrez]
PHST- 2017/06/24 06:00 [pubmed]
PHST- 2017/09/21 06:00 [medline]
PHST- 2017/06/22 00:00 [pmc-release]
AID - PONE-D-17-06521 [pii]
AID - 10.1371/journal.pone.0179460 [doi]
PST - epublish
SO  - PLoS One. 2017 Jun 22;12(6):e0179460. doi: 10.1371/journal.pone.0179460. 
      eCollection 2017.