PMID- 28642578
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20181231
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jun 22
TI  - Human endothelial cells secrete neurotropic factors to direct axonal growth of 
      peripheral nerves.
PG  - 4092
LID - 10.1038/s41598-017-04460-8 [doi]
LID - 4092
AB  - Understanding how nerves spontaneously innervate tissues or regenerate small 
      injuries is critical to enhance material-based interventions to regenerate large 
      scale, traumatic injuries. During embryogenesis, neural and vascular tissues form 
      interconnected, complex networks as a result of signaling between these tissue 
      types. Here, we report that human endothelial cells (HUVECs) secrete 
      brain-derived neurotrophic factor (BDNF), which significantly stimulated axonal 
      growth from chicken or rat dorsal root ganglia (DRGs). HUVEC-conditioned medium 
      was sufficient to enhance axonal growth, demonstrating that direct cell-cell 
      contact was not required. When BDNF was neutralized, there was a significant 
      reduction in axonal growth when incubated in HUVEC-conditioned medium and in 
      direct co-culture with HUVECs. These data show that HUVECs secrete neurotrophic 
      factors that significantly enhance axonal growth, and can inform future in vivo 
      studies to direct or pattern the angiogenic response in regenerating tissues to 
      encourage re-innervation.
FAU - Grasman, Jonathan M
AU  - Grasman JM
AUID- ORCID: 0000-0001-8289-9627
AD  - Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
FAU - Kaplan, David L
AU  - Kaplan DL
AD  - Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA. 
      david.kaplan@tufts.edu.
LA  - eng
GR  - F32 DE026058/DE/NIDCR NIH HHS/United States
GR  - P41 EB002520/EB/NIBIB NIH HHS/United States
GR  - R01 AR055993/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170622
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/*metabolism
MH  - Cytokines/biosynthesis/pharmacology
MH  - Endothelial Cells/*metabolism
MH  - Ganglia, Spinal/growth & development/metabolism
MH  - Human Umbilical Vein Endothelial Cells/metabolism
MH  - Humans
MH  - Nerve Growth Factors/*biosynthesis/pharmacology
MH  - *Neurogenesis/drug effects
MH  - Peripheral Nerves/*growth & development/*metabolism
PMC - PMC5481420
COIS- The authors declare that they have no competing interests.
EDAT- 2017/06/24 06:00
MHDA- 2019/01/01 06:00
PMCR- 2017/06/22
CRDT- 2017/06/24 06:00
PHST- 2016/12/06 00:00 [received]
PHST- 2017/05/16 00:00 [accepted]
PHST- 2017/06/24 06:00 [entrez]
PHST- 2017/06/24 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
PHST- 2017/06/22 00:00 [pmc-release]
AID - 10.1038/s41598-017-04460-8 [pii]
AID - 4460 [pii]
AID - 10.1038/s41598-017-04460-8 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jun 22;7(1):4092. doi: 10.1038/s41598-017-04460-8.