PMID- 28646332
OWN - NLM
STAT- MEDLINE
DCOM- 20180829
LR  - 20180829
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 40
IP  - 10
DP  - 2017 Oct
TI  - A rational approach for the improvement of biomass production and lipid profile 
      in cacao cell suspensions.
PG  - 1479-1492
LID - 10.1007/s00449-017-1805-z [doi]
AB  - Cocoa butter (CB) is produced in the seeds of Theobroma cacao representing 50% of 
      its dry weight. The lipid composition plays an important role in the 
      physicochemical, rheological, and sensory properties of the CB, making this fat a 
      valuable resource for the production of chocolates, cosmetics, and 
      pharmaceuticals. In this paper, are described experimental strategies used for a 
      rational improvement of biomass production and fatty acids in cacao cell 
      suspension cultures. First, the lipid profile in four cacao varieties is 
      characterized, and then, one variety is selected to induce cell suspensions using 
      a direct method without previous establishment of a callus phase. To improve 
      growth and total fat production in cell suspension cultures, modified DKW media 
      and newly designed media culture, based on the mineral concentrations of cacao 
      seeds (cacao biomass production, "CBP"), are analyzed and compared. In addition, 
      the effect of acetate in the lipid profile of cell suspensions is evaluated. 
      Ultrastructural histological analysis of lipid vesicles in cacao seeds and cell 
      suspensions is also performed. The results will show that it is feasible to 
      establish cacao suspensions without the calli step and increase the biomass 
      production by selecting a suitable cacao variety and tissue and also applying a 
      new culture media formulation. In addition, it is possible to synthesize fatty 
      acids in cell cultures and modify the lipid profile adding a precursor of the 
      novo biosynthesis of fatty acids such as the acetate. Transmission electronic 
      microscopy examinations and differential interference contrast microscopy 
      analysis will demonstrate that lipid vesicles are the main reserve substance in 
      both cacao seeds and cell suspensions.
FAU - Rua, Adriana Maria Gallego
AU  - Rua AMG
AD  - Universidad de Antioquia, Grupo de Biotecnologia, Calle 70 No 52-21, AA 1226, 
      Medellin, Colombia.
FAU - Rojas, Luisa Fernanda
AU  - Rojas LF
AUID- ORCID: 0000-0002-6462-4926
AD  - Universidad de Antioquia, Grupo de Biotecnologia, Escuela de Microbiologia, Calle 
      70 No 52-21, A.A 1226, Medellin, Colombia. lfernanda.rojas@udea.edu.co.
FAU - Trujillo, Aura Ines Urrea
AU  - Trujillo AIU
AD  - Universidad de Antioquia, Grupo de Biotecnologia, Calle 70 No 52-21, AA 1226, 
      Medellin, Colombia.
FAU - Zuleta, Oriana Parra
AU  - Zuleta OP
AD  - Universidad de Antioquia, Grupo de Biotecnologia, Calle 70 No 52-21, AA 1226, 
      Medellin, Colombia.
FAU - Alvarez, Cristian David Correa
AU  - Alvarez CDC
AD  - Instituto Tecnologico Metropolitano, Grupo de investigacion: Calidad, Metrologia 
      y Produccion, Calle 73 No 76A-354, Medellin, Colombia.
FAU - Garces, Lucia Atehortua
AU  - Garces LA
AD  - Universidad de Antioquia, Grupo de Biotecnologia, Calle 70 No 52-21, AA 1226, 
      Medellin, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20170623
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Membrane Lipids)
SB  - IM
MH  - *Biomass
MH  - *Cacao/cytology/metabolism
MH  - Cell Culture Techniques
MH  - Membrane Lipids/*metabolism
MH  - Plant Cells/*metabolism
OTO - NOTNLM
OT  - Biomass
OT  - Cacao
OT  - Cell suspensions
OT  - Cocoa butter
OT  - Fatty acids
OT  - Lipid vesicles
EDAT- 2017/06/25 06:00
MHDA- 2018/08/30 06:00
CRDT- 2017/06/25 06:00
PHST- 2016/11/25 00:00 [received]
PHST- 2017/06/14 00:00 [accepted]
PHST- 2017/06/25 06:00 [pubmed]
PHST- 2018/08/30 06:00 [medline]
PHST- 2017/06/25 06:00 [entrez]
AID - 10.1007/s00449-017-1805-z [pii]
AID - 10.1007/s00449-017-1805-z [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2017 Oct;40(10):1479-1492. doi: 
      10.1007/s00449-017-1805-z. Epub 2017 Jun 23.