PMID- 28646704
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR  - 20200930
IS  - 1873-2690 (Electronic)
IS  - 0981-9428 (Linking)
VI  - 118
DP  - 2017 Sep
TI  - Location and characterization of lignin in tracheid cell walls of radiata pine 
      (Pinus radiata D. Don) compression woods.
PG  - 187-198
LID - S0981-9428(17)30203-6 [pii]
LID - 10.1016/j.plaphy.2017.06.012 [doi]
AB  - Tilted stems of softwoods form compression wood (CW) and opposite wood (OW) on 
      their lower and upper sides, respectively. More is known about the most severe 
      form of CW, severe CW (SCW), but mild CWs (MCWs) also occur widely. Two grades of 
      MCWs, MCW1 and MCW2, as well as SCW and OW were identified in the stems of 
      radiata pine (Pinus radiata) that had been slightly tilted. The four wood types 
      were identified by the distribution of lignin in the tracheid walls determined by 
      fluorescence microscopy. A solution of the fluorescent dye acridine orange (AO) 
      (0.02% at pH 6 or 7) was shown to metachromatically stain the tracheid walls and 
      can also be used to determine lignin distribution. The lignified walls fluoresced 
      orange to yellow depending on the lignin concentration. Microscopically 
      well-characterized discs (0.5 mm diameter) of the wood types were used to 
      determine lignin concentrations and lignin monomer compositions using the acetyl 
      bromide method and thioacidolysis, respectively. Lignin concentration and the 
      proportion of p-hydroxyphenyl units (H-units) relative to guaiacyl (G-units) 
      increased with CW severity, with <1% H-units in OW and up to 14% in SCW. Lignin 
      H-units can be used as a marker for CW and CW severity. Similar discs were also 
      examined by Raman and FTIR micro-spectroscopies coupled with principal component 
      analysis (PCA) to determine if these techniques can be used to differentiate the 
      four different wood types. Both techniques were able to do this, particularly 
      Raman micro-spectroscopy.
CI  - Copyright (c) 2017 Elsevier Masson SAS. All rights reserved.
FAU - Zhang, Miao
AU  - Zhang M
AD  - School of Biological Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      mzha084@aucklanduni.ac.nz.
FAU - Lapierre, Catherine
AU  - Lapierre C
AD  - INRA, Institut Jean-Pierre Bourgin (IJPB), UMR1318, Saclay Plant Sciences, Route 
      de St-Cyr, 78000 Versailles, France; Agroparistech, Institut Jean-Pierre Bourgin 
      (IJPB), UMR1318, Saclay Plant Sciences, Route de St-Cyr, 78000 Versailles, 
      France. Electronic address: catherine.lapierre@inra.fr.
FAU - Nouxman, Noor Liyana
AU  - Nouxman NL
AD  - School of Biological Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      nnou003@aucklanduni.ac.nz.
FAU - Nieuwoudt, Michel K
AU  - Nieuwoudt MK
AD  - School of Chemical Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      m.nieuwoudt@auckland.ac.nz.
FAU - Smith, Bronwen G
AU  - Smith BG
AD  - School of Chemical Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      b.smith@auckland.ac.nz.
FAU - Chavan, Ramesh R
AU  - Chavan RR
AD  - School of Biological Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      r.chavan@auckland.ac.nz.
FAU - McArdle, Brian H
AU  - McArdle BH
AD  - Department of Statistics, The University of Auckland, Private Bag 92019, Auckland 
      Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      b.mcardle@auckland.ac.nz.
FAU - Harris, Philip J
AU  - Harris PJ
AD  - School of Biological Sciences, The University of Auckland, Private Bag 92019, 
      Auckland Mail Centre, Auckland 1142, New Zealand. Electronic address: 
      p.harris@auckland.ac.nz.
LA  - eng
PT  - Journal Article
DEP - 20170615
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 9005-53-2 (Lignin)
SB  - IM
MH  - Cell Wall/*metabolism
MH  - Lignin/*metabolism
MH  - Pinus/cytology/*metabolism
MH  - Wood/cytology/*metabolism
OTO - NOTNLM
OT  - Acridine orange
OT  - Compression wood
OT  - FTIR micro-spectroscopy
OT  - Lignin
OT  - Principal component analysis (PCA)
OT  - Raman micro-spectroscopy
OT  - Thioacidolysis
EDAT- 2017/06/25 06:00
MHDA- 2017/12/22 06:00
CRDT- 2017/06/25 06:00
PHST- 2017/04/24 00:00 [received]
PHST- 2017/06/12 00:00 [revised]
PHST- 2017/06/12 00:00 [accepted]
PHST- 2017/06/25 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
PHST- 2017/06/25 06:00 [entrez]
AID - S0981-9428(17)30203-6 [pii]
AID - 10.1016/j.plaphy.2017.06.012 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2017 Sep;118:187-198. doi: 10.1016/j.plaphy.2017.06.012. 
      Epub 2017 Jun 15.