PMID- 28647881
OWN - NLM
STAT- MEDLINE
DCOM- 20181022
LR  - 20190318
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 24
IP  - 22
DP  - 2017 Aug
TI  - Alterations in soil microbial communities caused by treatments with penicillin or 
      neomycin.
PG  - 18651-18662
LID - 10.1007/s11356-017-9530-3 [doi]
AB  - Antibiotic residues in soils can lead to serious health risk and ecological 
      hazards. In this study, the effects of penicillin and neomycin, two antibiotics 
      widely used in animal production, were investigated on soil bacterial 
      communities. Changes in the community structure were monitored using three 16S 
      ribosomal DNA (rDNA) polymerase chain reaction-based approaches, including 
      denaturing gradient gel electrophoresis (DGGE), amplified rDNA restriction 
      analysis (ARDRA), and terminal-restriction fragment length polymorphism (T-RFLP) 
      analysis. The prominent DGGE bands were excised from gels and sequenced, and the 
      data indicated the prevalence of Gammaproteobacteria in the soils. The total soil 
      bacterial community, including uncultured bacteria, exhibited a higher diversity 
      than that of cultured bacteria. Some microbial strains were capable of surviving 
      and even subsisting on penicillin or neomycin. We also observed toxic effects of 
      the antibiotics on the indigenous soil bacterial communities since some genotypes 
      disappeared after the treatments (e.g., Pseudomonas sp., Stenotrophomonas sp., 
      Salinimonas, and uncultured Acinetobacter sp.). The implications of these 
      findings are that the functions of soil bacterial communities may be negatively 
      affected if key microbial community members are lost.
FAU - Zhang, Qichun
AU  - Zhang Q
AD  - Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Key 
      Laboratory of Environment Remediation and Ecological Health, Ministry of 
      Education, Zhejiang University, Hangzhou, 310058, China. qczhang@zju.edu.cn.
FAU - Kang, Ouyang
AU  - Kang O
AD  - College of Animal Science and Technology, Guangxi University, Nanning, 530001, 
      China.
FAU - Jabeen, Salma
AU  - Jabeen S
AD  - Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Key 
      Laboratory of Environment Remediation and Ecological Health, Ministry of 
      Education, Zhejiang University, Hangzhou, 310058, China.
FAU - Dick, Warren A
AU  - Dick WA
AD  - The Ohio State University, Wooster, OH, 44691, USA.
LA  - eng
GR  - 41401266/The National Natural Science Foundation of China/
GR  - 2016YFD0801103/The National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20170625
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (DNA, Bacterial)
RN  - 0 (Penicillins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - I16QD7X297 (Neomycin)
SB  - IM
MH  - Bacteria/classification/*drug effects/genetics
MH  - *Biodiversity
MH  - DNA, Bacterial/genetics
MH  - Microbial Consortia/*drug effects
MH  - Neomycin/*toxicity
MH  - Penicillins/*toxicity
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Soil Microbiology
MH  - Soil Pollutants/toxicity
OTO - NOTNLM
OT  - ARDRA
OT  - DGGE
OT  - Neomycin
OT  - Penicillin
OT  - Soil microbial community
OT  - T-RFLP
EDAT- 2017/06/26 06:00
MHDA- 2018/10/23 06:00
CRDT- 2017/06/26 06:00
PHST- 2017/02/23 00:00 [received]
PHST- 2017/06/12 00:00 [accepted]
PHST- 2017/06/26 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
PHST- 2017/06/26 06:00 [entrez]
AID - 10.1007/s11356-017-9530-3 [pii]
AID - 10.1007/s11356-017-9530-3 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2017 Aug;24(22):18651-18662. doi: 
      10.1007/s11356-017-9530-3. Epub 2017 Jun 25.