PMID- 2864821 OWN - NLM STAT- MEDLINE DCOM- 19851114 LR - 20051117 IS - 0001-723X (Print) IS - 0001-723X (Linking) VI - 29 IP - 3 DP - 1985 May TI - Inhibition by Norakin (triperiden) of Sindbis virus infection in mice. PG - 209-15 AB - Intranasal (i. n.) infection with 10 LD50 of Sindbis virus caused acute encephalomyelitis and death in ABD2F1 mice 3-7 days post infection (p.i.). Histologic lesions were found in the CNS, pancreas. liver, parotid glands, exorbital lacrimal glands, lymphoid organs and kidneys. Repeated oral administration of the anticholinergic anti-Parkinson drug Norakin protected infected animals from death in a dose-dependent manner when treatment was started prior to but not after virus inoculation. The maximum protective effect was achieved when the drug was administered twice daily at doses of 2.5 or 5.0 mg/kg body mass for at least 56 hr; single injections of the full daily dose were ineffective. Daily doses of greater than or equal to 25 mg/kg body mass had a reduced protective effect or failed to prevent mortality. Administration of Norakin up to doses of 300 mg/kg body mass per day to noninfected ABD2F1 mice were tolerated without obvious clinical or histological signs of illness over a period of 104 hr. Replication of sindbis virus in BHK 21/C13 cells was not inhibited by Norakin concentrations up to 10 micrograms/ml. In Mengo virus-infected mice Norakin did not exert any protective effect within the range of 1.25-50.0 mg/kg body mass when treatment started 1 hr before infection and has been continued twice daily over a period of 104 hr. FAU - Veckenstedt, A AU - Veckenstedt A FAU - Guttner, J AU - Guttner J FAU - Schroeder, C AU - Schroeder C LA - eng PT - Journal Article PL - Slovakia TA - Acta Virol JT - Acta virologica JID - 0370401 RN - 0 (Antiparkinson Agents) RN - 0 (Antiviral Agents) RN - 0 (Piperidines) RN - 14617-17-5 (triperiden) SB - IM MH - Animals MH - Antiparkinson Agents MH - *Antiviral Agents MH - Cells, Cultured MH - Cricetinae MH - Enterovirus Infections/prevention & control MH - Mengovirus MH - Mice MH - Piperidines/pharmacology/*therapeutic use MH - Sindbis Virus MH - Togaviridae Infections/*prevention & control MH - Virus Replication/drug effects EDAT- 1985/05/01 00:00 MHDA- 1985/05/01 00:01 CRDT- 1985/05/01 00:00 PHST- 1985/05/01 00:00 [pubmed] PHST- 1985/05/01 00:01 [medline] PHST- 1985/05/01 00:00 [entrez] PST - ppublish SO - Acta Virol. 1985 May;29(3):209-15.