PMID- 28649655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2374-4677 (Print)
IS  - 2374-4677 (Electronic)
IS  - 2374-4677 (Linking)
VI  - 3
DP  - 2017
TI  - Gene expression in breast and adipose tissue after 12 months of weight loss and 
      vitamin D supplementation in postmenopausal women.
PG  - 15
LID - 10.1038/s41523-017-0019-5 [doi]
LID - 15
AB  - Adipose tissue is involved in the etiology of postmenopausal breast cancer, 
      possibly through increased sex steroid hormone production, inflammation, and 
      altered adipokines. Vitamin D may affect these pathways but its effect on gene 
      expression in different tissues has not been examined. Within a double-blind, 
      12-month placebo-controlled randomized trial, we compared 2000 IU/day oral 
      vitamin D(3) supplementation (N = 39) vs. placebo (N = 40) on the expression of 5 
      genes in breast and adipose tissue in overweight/obese postmenopausal women 
      (50-75 years). All participants had serum 25-hydroxyvitamin D (25(OH)D) 
      levels >/= 10-<32 ng/mL ("insufficient") and concurrently completed a behavioral 
      weight loss program. Random periareolar fine needle aspiration (RPFNA) and 
      abdominal subcutaneous adipose tissue biopsies were performed at baseline and 12 
      months. Changes in expression of aromatase (CYP19A1), peroxisome 
      proliferator-activated receptor gamma (PPARG), adiponectin (ADIPOQ), 
      monocyte-chemoattractant protein 1 (MCP-1), and vitamin D receptor (VDR) were 
      analyzed by qRT-PCR. Compared to placebo, 2000 IU vitamin D did not show 
      significant effects on gene expression in breast or adipose tissue. Replete women 
      (i.e., 25(OH)D >/= 32 ng/mL; N = 17) showed a small decrease in MCP-1 expression 
      compared to an increase among women who remained 'insufficient' despite 
      supplementation (N = 12) (Replete:-1.6% vs. Non-replete: 61.2%, p = 0.015) in 
      breast, but not adipose tissue. No statistically significant differences in gene 
      expression were detected according to degree of weight loss. Vitamin D repletion 
      during weight loss may have different effects on gene expression in breast and 
      adipose tissue. Further research on the localized effects of vitamin D is needed 
      to determine its effect on breast cancer risk.
FAU - Mason, Caitlin
AU  - Mason C
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
FAU - Wang, Lei
AU  - Wang L
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
FAU - Duggan, Catherine
AU  - Duggan C
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
FAU - Imayama, Ikuyo
AU  - Imayama I
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
FAU - Thomas, Sushma S
AU  - Thomas SS
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
FAU - Wang, Ching-Yun
AU  - Wang CY
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
AD  - Department of Biostatistics, University of Washington, Seattle, WA USA. GRID: 
      grid.34477.33
FAU - Korde, Larissa A
AU  - Korde LA
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
AD  - Department of Medicine, University of Washington, Seattle, WA USA. GRID: 
      grid.34477.33
FAU - McTiernan, Anne
AU  - McTiernan A
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA USA. GRID: grid.270240.3
AD  - Department of Epidemiology, University of Washington, Seattle, WA USA. GRID: 
      grid.34477.33
LA  - eng
GR  - P30 CA015704/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20170421
PL  - United States
TA  - NPJ Breast Cancer
JT  - NPJ breast cancer
JID - 101674891
PMC - PMC5460115
COIS- The authors have no competing interests to disclose.
EDAT- 2017/06/27 06:00
MHDA- 2017/06/27 06:01
PMCR- 2017/04/21
CRDT- 2017/06/27 06:00
PHST- 2016/03/07 00:00 [received]
PHST- 2017/03/20 00:00 [revised]
PHST- 2017/03/27 00:00 [accepted]
PHST- 2017/06/27 06:00 [entrez]
PHST- 2017/06/27 06:00 [pubmed]
PHST- 2017/06/27 06:01 [medline]
PHST- 2017/04/21 00:00 [pmc-release]
AID - 19 [pii]
AID - 10.1038/s41523-017-0019-5 [doi]
PST - epublish
SO  - NPJ Breast Cancer. 2017 Apr 21;3:15. doi: 10.1038/s41523-017-0019-5. eCollection 
      2017.