PMID- 28650775
OWN - NLM
STAT- MEDLINE
DCOM- 20170919
LR  - 20180103
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 188
IP  - 3
DP  - 2017 Sep
TI  - Urine Interleukin-18 (IL-18) as a Biomarker of Total-Body Irradiation: A 
      Preliminary Study in Nonhuman Primates.
PG  - 325-334
LID - 10.1667/RR14768.1 [doi]
AB  - We have reported that circulating IL-18 can be used as a radiation biomarker in 
      mice, minipigs and nonhuman primates (NHPs, Macaca mulatta). Here, we report the 
      levels of IL-18 in individual NHP's urine before and at 6 h-7 days after 5.0, 6.5 
      and 8.5 Gy (60)Co total-body irradiation (TBI) using enzyme linked immunosorbent 
      assay (ELISA). Six animals (3.5-5.5 kg, 3-4 years old) per radiation dose were 
      investigated. Correlation values between urine IL-18 and blood cell counts and 
      serum chemistry parameters including lactate dehydrogenase (LDH), lipase, and 
      serum total protein (TP), as well as between urine IL-18 and 60-day survival, 
      were analyzed. Our data, to the best of our knowledge, for the first time, 
      demonstrate that concentrations of urine IL-18 from irradiated NHPs were 
      increased in a radiation dose-dependent manner compared to pre-TBI levels in 
      samples from these animal (N = 18, 11.02 +/- 1.3 pg/ml). A 5.0 Gy low dose of 
      radiation ( approximately LD(10/60)) did not increase urine IL-18 levels. In contrast, 
      high-dose TBI significantly increased urine IL-18 at day 1 to day 5 in a 
      bell-shaped time course, reaching a peak of 5- to 10-fold of control levels on 
      day 3 after 6.5 Gy ( approximately LD(50/60)) and 8.5 Gy ( approximately LD(90/60)), respectively. 
      Statistical analysis using receiver operator characteristic (ROC) and MultiROC 
      analysis indicated that white blood cell and platelet counts, serum LDH, lipase 
      and TP, when combined with urine IL-18, provide discriminatory predictors of 
      total-body radiation injury with a very high ROC area of 0.98. Urine IL-18 
      measurement, as an early prognostic indicator of survival, may facilitate rapid 
      detection of lethal doses of radiation, based on the currently available data 
      set.
FAU - Xiao, Mang
AU  - Xiao M
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
FAU - Bolduc, David L
AU  - Bolduc DL
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
FAU - Li, XiangHong
AU  - Li X
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
FAU - Cui, Wanchang
AU  - Cui W
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
FAU - Hieber, Kevin P
AU  - Hieber KP
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
FAU - Bunger, Rolf
AU  - Bunger R
AD  - b   Consultant, McLean, Virginia.
FAU - Ossetrova, Natalia I
AU  - Ossetrova NI
AD  - a   Armed Forces Radiobiology Research Institute, Uniformed Services University 
      of the Health Sciences, Bethesda, Maryland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170626
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Biomarkers)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods
MH  - Biomarkers/urine
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - Humans
MH  - Interleukin-18/*urine
MH  - Macaca mulatta
MH  - Male
MH  - Pilot Projects
MH  - Radiation Dosage
MH  - Radiation Exposure/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Whole-Body Counting/*methods
EDAT- 2017/06/27 06:00
MHDA- 2017/09/20 06:00
CRDT- 2017/06/27 06:00
PHST- 2017/06/27 06:00 [pubmed]
PHST- 2017/09/20 06:00 [medline]
PHST- 2017/06/27 06:00 [entrez]
AID - 10.1667/RR14768.1 [doi]
PST - ppublish
SO  - Radiat Res. 2017 Sep;188(3):325-334. doi: 10.1667/RR14768.1. Epub 2017 Jun 26.