PMID- 28651931
OWN - NLM
STAT- MEDLINE
DCOM- 20170818
LR  - 20171129
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 490
IP  - 3
DP  - 2017 Aug 26
TI  - Upregulated long non-coding RNA LOC90784 promotes cell proliferation and invasion 
      and is associated with poor clinical features in HCC.
PG  - 920-926
LID - S0006-291X(17)31272-X [pii]
LID - 10.1016/j.bbrc.2017.06.141 [doi]
AB  - A growing amount of literature has indicated that long non-coding RNAs (lncRNAs) 
      are important factors in hepatocellular carcinoma (HCC) progression. However, the 
      significance of lncRNAs in the progression and prognosis of liver cancer is 
      largely unknown. In the present study, upregulated lncRNA LOC90784 was identified 
      through integrative analysis of GSE58043 and GSE55191. Furthermore, associations 
      between LOC90784 expression and the clinicopathological characteristics of 
      patients were analyzed with a validated cohort 1 and the Cancer Genome Atlas 
      (TCGA) cohort 2. We investigated the mechanisms by which this highly expressed 
      lncRNA promotes HCC proliferation, invasion and migration via qRT-PCR, 
      fluorescence in situ hybridization (FISH) staining, siRNA transfection, cell 
      proliferation assays, Transwell and colony formation assays, flow cytometry 
      analysis and Western blot. The results showed that LOC90784 expression levels 
      were significantly higher in HCC cell lines and tissues and mainly localized in 
      the cytoplasm. Knockdown of lncRNA LOC90784 expression inhibited proliferation 
      and induced apoptosis and cell cycle arrest by promoting Bax and repressing CDK4 
      and Cyclin D1 protein expression; it also inhibited invasion and migration by 
      repressing MMP2 and MMP9 expression in HCC cells. LOC90784 overexpression was 
      associated with poor clinical features in the two cohorts and poor overall 
      survival rates in HCC patients with clear resection margins (R0) in cohort 2. 
      These results indicated that LOC90784 upregulation may be a critical oncogene and 
      potential new biomarker in HCC.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Xu, Jian-Hua
AU  - Xu JH
AD  - Department of Hepatobiliary Surgery, Daping Hospital & Research Institute of 
      Surgery, Third Military Medical University, Chongqing 400042, PR China.
FAU - Chang, Wei-Hua
AU  - Chang WH
AD  - Department of Hepatobiliary Surgery, Daping Hospital & Research Institute of 
      Surgery, Third Military Medical University, Chongqing 400042, PR China.
FAU - Fu, Hang-Wei
AU  - Fu HW
AD  - Department of Hepatobiliary Surgery, Daping Hospital & Research Institute of 
      Surgery, Third Military Medical University, Chongqing 400042, PR China.
FAU - Shu, Wei-Qun
AU  - Shu WQ
AD  - Department of Environmental Hygiene, College of Preventive Medicine, Third 
      Military Medical University, Chongqing 400038, PR China.
FAU - Yuan, Tao
AU  - Yuan T
AD  - Department of Hepatobiliary Surgery, Daping Hospital & Research Institute of 
      Surgery, Third Military Medical University, Chongqing 400042, PR China. 
      Electronic address: yuantaotmmu@163.com.
FAU - Chen, Ping
AU  - Chen P
AD  - Department of Hepatobiliary Surgery, Daping Hospital & Research Institute of 
      Surgery, Third Military Medical University, Chongqing 400042, PR China. 
      Electronic address: chenpingsyd@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170624
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long non-coding RNA LOC90784, human)
SB  - IM
MH  - Apoptosis
MH  - Carcinoma, Hepatocellular/genetics/*pathology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Liver/metabolism/*pathology
MH  - Liver Neoplasms/genetics/*pathology
MH  - Neoplasm Invasiveness/genetics/*pathology
MH  - RNA, Long Noncoding/*genetics
MH  - Up-Regulation
OTO - NOTNLM
OT  - Biomarker
OT  - Hepatocellular carcinoma
OT  - LOC90784
OT  - Long non-coding RNA
OT  - Progression
EDAT- 2017/06/28 06:00
MHDA- 2017/08/19 06:00
CRDT- 2017/06/28 06:00
PHST- 2017/06/08 00:00 [received]
PHST- 2017/06/19 00:00 [revised]
PHST- 2017/06/23 00:00 [accepted]
PHST- 2017/06/28 06:00 [pubmed]
PHST- 2017/08/19 06:00 [medline]
PHST- 2017/06/28 06:00 [entrez]
AID - S0006-291X(17)31272-X [pii]
AID - 10.1016/j.bbrc.2017.06.141 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Aug 26;490(3):920-926. doi: 
      10.1016/j.bbrc.2017.06.141. Epub 2017 Jun 24.