PMID- 28652211
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20211204
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 89
DP  - 2017 Aug
TI  - CHOP negatively regulates Polo-like kinase 2 expression via recruiting C/EBPalpha to 
      the upstream-promoter in human osteosarcoma cell line during ER stress.
PG  - 207-215
LID - S1357-2725(17)30150-4 [pii]
LID - 10.1016/j.biocel.2017.06.012 [doi]
AB  - Polo-like kinase 2 (Plk2) is a member of the serine/threonine protein kinase 
      family involved in cell-cycle regulation and cellular response to stresses. 
      However, the alteration of Plk2 in response to endoplasmic reticulum (ER) stress 
      has not been well described. In the present study, we focused on the regulation 
      of Plk2 regulation in response to ER stress. Plk2 expression was dramatically 
      decreased under ER stress induced by brefeldin A (BFA), thapsigargin (TG), or 
      tunicamycin (TM), and this down regulation of Plk2 expression was dependent on 
      activating transcription factor 4 (ATF4) and C/EBP homology protein (CHOP). 
      Luciferase activity analysis of the truncated Plk2 promoter indicated that 
      regions from -2506 to -1806 and from -1002 to -830 of the Plk2 promoter were 
      sensitive to BFA. Additionally, ChIP and ChIP Re-IP assays showed that CHOP and 
      C/EBPalpha were assembled on the same region of Plk2 promoter. Notably, we identified 
      two C/EBPalpha responsive elements at positions -2002 and -948, to which C/EBPalpha or 
      CHOP binding was enhanced by BFA under in vitro and in vivo conditions. Finally, 
      overexpression of Plk2 inhibits cell apoptosis and promotes cell proliferation in 
      response to ER stress. In summary, these results demonstrated that ER stress 
      plays a crucial role in Plk2 expression. CHOP may up-regulate DNA-binding 
      affinities after BFA treatment, via recruiting C/EBPalpha to the upstream-promoter of 
      Plk2. These findings may contribute to the understanding of the molecular 
      mechanism of Plk2 regulation.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Shen, Tao
AU  - Shen T
AD  - Department of Orthopedic Surgery, Shengjing Hospital, China Medical University, 
      Shenyang 110004, People's Republic of China. Electronic address: 
      shent@sj-hospital.org.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public 
      Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China 
      Medical University, Shenyang 110001, People's Republic of China.
FAU - Chen, Zhiguang
AU  - Chen Z
AD  - Department of Orthopedic Surgery, Shengjing Hospital, China Medical University, 
      Shenyang 110004, People's Republic of China.
FAU - Liang, Shuang
AU  - Liang S
AD  - Department of Laboratory Medicine & Pathology, University of Minnesota, 
      Minneapolis, MN 55455, USA.
FAU - Guo, Zhouyang
AU  - Guo Z
AD  - Department of Orthopedic Surgery, Shengjing Hospital, China Medical University, 
      Shenyang 110004, People's Republic of China.
FAU - Wang, Ping
AU  - Wang P
AD  - Department of Neurobiology, College of Basic Medicine, China Medical University, 
      Shenyang 110001, People's Republic of China.
FAU - Wu, Qijun
AU  - Wu Q
AD  - Department of Clinical Epidemiology, Shengjing Hospital, China Medical 
      University, Shenyang 110004, People's Republic of China.
FAU - Ba, Gen
AU  - Ba G
AD  - Department of Orthopedic Surgery, Shengjing Hospital, China Medical University, 
      Shenyang 110004, People's Republic of China.
FAU - Fu, Qin
AU  - Fu Q
AD  - Department of Orthopedic Surgery, Shengjing Hospital, China Medical University, 
      Shenyang 110004, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20170624
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (ATF4 protein, human)
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 145891-90-3 (Activating Transcription Factor 4)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - EC 2.7.11.- (PLK2 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Activating Transcription Factor 4/metabolism
MH  - Apoptosis/genetics
MH  - CCAAT-Enhancer-Binding Protein-alpha/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Endoplasmic Reticulum Stress/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mutagenesis
MH  - Nucleotide Motifs/genetics
MH  - Osteosarcoma/*pathology
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - Protein Transport
MH  - Transcription Factor CHOP/*metabolism
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - C/EBPalpha
OT  - CHOP
OT  - ER stress
OT  - Expression regulation
OT  - Plk2
EDAT- 2017/06/28 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/06/28 06:00
PHST- 2016/12/22 00:00 [received]
PHST- 2017/06/09 00:00 [revised]
PHST- 2017/06/22 00:00 [accepted]
PHST- 2017/06/28 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/06/28 06:00 [entrez]
AID - S1357-2725(17)30150-4 [pii]
AID - 10.1016/j.biocel.2017.06.012 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2017 Aug;89:207-215. doi: 10.1016/j.biocel.2017.06.012. 
      Epub 2017 Jun 24.