PMID- 28654176
OWN - NLM
STAT- MEDLINE
DCOM- 20180130
LR  - 20210925
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 19
IP  - 10
DP  - 2017 Oct
TI  - Multiple Pseudomonas species secrete exolysin-like toxins and provoke 
      Caspase-1-dependent macrophage death.
PG  - 4045-4064
LID - 10.1111/1462-2920.13841 [doi]
AB  - Pathogenic bacteria secrete protein toxins that provoke apoptosis or necrosis of 
      eukaryotic cells. Here, we developed a live-imaging method, based on 
      incorporation of a DNA-intercalating dye into membrane-damaged host cells, to 
      study the kinetics of primary bone marrow-derived macrophages (BMDMs) mortality 
      induced by opportunistic pathogen Pseudomonas aeruginosa expressing either Type 
      III Secretion System (T3SS) toxins or the pore-forming toxin, Exolysin (ExlA). We 
      found that ExlA promotes the activation of Caspase-1 and maturation of 
      interleukin-1beta. BMDMs deficient for Caspase-1 and Caspase-11 were resistant to 
      ExlA-induced death. Furthermore, by using KO BMDMs, we determined that the 
      upstream NLRP3/ASC complex leads to the Caspase-1 activation. We also 
      demonstrated that Pseudomonas putida and Pseudomonas protegens and the Drosophila 
      pathogen Pseudomonas entomophila, which naturally express ExlA-like toxins, are 
      cytotoxic toward macrophages and provoke the same type of pro-inflammatory death 
      as does ExlA(+) P. aeruginosa. These results demonstrate that ExlA-like toxins of 
      two-partner secretion systems from diverse Pseudomonas species activate the NLRP3 
      inflammasome and provoke inflammatory pyroptotic death of macrophages.
CI  - (c) 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Basso, Pauline
AU  - Basso P
AD  - CNRS-ERL5261, INSERM, U1036, CEA, Bacterial Pathogenesis and Cellular Responses, 
      Biosciences and Biotechnology Institute of Grenoble, University Grenoble Alpes, 
      France.
FAU - Wallet, Pierre
AU  - Wallet P
AD  - CIRI, Centre International de Recherche en Infectiologie, INSERM, U1111, 
      Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Superieure de 
      Lyon, Univ Lyon, Lyon, F-69007, France.
FAU - Elsen, Sylvie
AU  - Elsen S
AD  - CNRS-ERL5261, INSERM, U1036, CEA, Bacterial Pathogenesis and Cellular Responses, 
      Biosciences and Biotechnology Institute of Grenoble, University Grenoble Alpes, 
      France.
FAU - Soleilhac, Emmanuelle
AU  - Soleilhac E
AD  - CMBA Platform, Biosciences and Biotechnology Institute of Grenoble, University 
      Grenoble Alpes, CEA, INSERM; Genetics & Chemogenomics, France.
FAU - Henry, Thomas
AU  - Henry T
AD  - CIRI, Centre International de Recherche en Infectiologie, INSERM, U1111, 
      Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Superieure de 
      Lyon, Univ Lyon, Lyon, F-69007, France.
FAU - Faudry, Eric
AU  - Faudry E
AD  - CNRS-ERL5261, INSERM, U1036, CEA, Bacterial Pathogenesis and Cellular Responses, 
      Biosciences and Biotechnology Institute of Grenoble, University Grenoble Alpes, 
      France.
FAU - Attree, Ina
AU  - Attree I
AD  - CNRS-ERL5261, INSERM, U1036, CEA, Bacterial Pathogenesis and Cellular Responses, 
      Biosciences and Biotechnology Institute of Grenoble, University Grenoble Alpes, 
      France.
LA  - eng
GR  - 311542/ERC_/European Research Council/International
PT  - Journal Article
DEP - 20170726
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (pseudomonas exoprotein A protein, Pseudomonas aeruginosa)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bacterial Proteins/toxicity
MH  - Bacterial Toxins/*toxicity
MH  - Bone Marrow Cells
MH  - Caspase 1/*metabolism
MH  - *Cell Death
MH  - Inflammasomes
MH  - Interleukin-1beta/metabolism
MH  - Macrophages/*microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pseudomonas/metabolism/*pathogenicity
EDAT- 2017/06/28 06:00
MHDA- 2018/01/31 06:00
CRDT- 2017/06/28 06:00
PHST- 2017/04/25 00:00 [received]
PHST- 2017/06/02 00:00 [revised]
PHST- 2017/06/19 00:00 [accepted]
PHST- 2017/06/28 06:00 [pubmed]
PHST- 2018/01/31 06:00 [medline]
PHST- 2017/06/28 06:00 [entrez]
AID - 10.1111/1462-2920.13841 [doi]
PST - ppublish
SO  - Environ Microbiol. 2017 Oct;19(10):4045-4064. doi: 10.1111/1462-2920.13841. Epub 
      2017 Jul 26.