PMID- 28654282
OWN - NLM
STAT- MEDLINE
DCOM- 20170811
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 65
IP  - 30
DP  - 2017 Aug 2
TI  - The Reduction in the IgE-Binding Ability of beta-Lactoglobulin by Dynamic 
      High-Pressure Microfluidization Coupled with Glycation Treatment Revealed by 
      High-Resolution Mass Spectrometry.
PG  - 6179-6187
LID - 10.1021/acs.jafc.7b00934 [doi]
AB  - Our previous study indicated that pretreatment by dynamic high-pressure 
      microfluidization (DHPM) and glycation with galactose was a promising method for 
      decreasing the immunoglobulin E (IgE)-binding ability of beta-lactoglobulin (beta-LG). 
      In this work, the conformational alteration of beta-LG subjected to DHPM and 
      glycation treatment was investigated in relation to IgE-binding ability by 
      orbitrap mass spectrometry. After DHPM pretreatment, lower IgE-binding ability of 
      glycated beta-LG was observed with increasing pressures. Prior to DHPM pretreatment, 
      11 glycated sites were identified, while the number of glycation sites was 
      increased to 12 after pretreatment. However, there was no significant difference 
      of the glycation sites at the pressures of 50, 100, and 200 MPa, respectively. 
      Average degree of substitution per peptide molecule of beta-LG (DSP) was 
      investigated to assess the degree of glycation per glycation site. All of the 
      samples pretreated by DHPM exhibited a higher glycation level than those without 
      DHPM pretreatment. The shielding effects of epitopes owing to glycation 
      contributed to the reduction of IgE-binding capacity. Orbitrap mass spectrometry 
      could provide a comprehensive understanding of the nature of protein glycation.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
FAU - Tu, Zongcai
AU  - Tu Z
AUID- ORCID: 0000-0001-8877-4681
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
AD  - State Key Laboratory of Food Science and Technology, Nanchang University , 
      Nanchang 330047, China.
FAU - Wang, Hui
AU  - Wang H
AUID- ORCID: 0000-0002-5538-1838
AD  - State Key Laboratory of Food Science and Technology, Nanchang University , 
      Nanchang 330047, China.
FAU - Zhang, Qiuting
AU  - Zhang Q
AD  - College of Ginling, Nanjing Normal University , Nanjing 210097, China.
FAU - Zhang, Lu
AU  - Zhang L
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
FAU - Sha, Xiaomei
AU  - Sha X
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
FAU - Huang, Tao
AU  - Huang T
AD  - State Key Laboratory of Food Science and Technology, Nanchang University , 
      Nanchang 330047, China.
FAU - Ma, Da
AU  - Ma D
AD  - State Key Laboratory of Food Science and Technology, Nanchang University , 
      Nanchang 330047, China.
FAU - Pang, Juanjuan
AU  - Pang J
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
FAU - Yang, Ping
AU  - Yang P
AD  - Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, 
      Ministry of Education , Nanchang 330022, China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20170719
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Lactoglobulins)
RN  - 0 (Peptides)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Glycosylation
MH  - Immunoglobulin E/*chemistry/metabolism
MH  - Lactoglobulins/*chemistry/metabolism
MH  - Mass Spectrometry/*methods
MH  - Microfluidics
MH  - Peptides/chemistry/metabolism
MH  - Pressure
MH  - Protein Binding
MH  - Protein Conformation
OTO - NOTNLM
OT  - IgE-binding ability
OT  - dynamic high-pressure microfluidization coupled with glycation
OT  - mass spectrometry
OT  - beta-lactoglobulin
EDAT- 2017/06/28 06:00
MHDA- 2017/08/12 06:00
CRDT- 2017/06/28 06:00
PHST- 2017/06/28 06:00 [pubmed]
PHST- 2017/08/12 06:00 [medline]
PHST- 2017/06/28 06:00 [entrez]
AID - 10.1021/acs.jafc.7b00934 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2017 Aug 2;65(30):6179-6187. doi: 10.1021/acs.jafc.7b00934. 
      Epub 2017 Jul 19.