PMID- 28656302
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR  - 20190221
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 40
IP  - 3
DP  - 2017 Sep
TI  - Inactivation of MSH3 by promoter methylation correlates with primary tumor stage 
      in nasopharyngeal carcinoma.
PG  - 673-678
LID - 10.3892/ijmm.2017.3044 [doi]
AB  - The aim of this study was to investigate the inactivation of the MutS homolog 
      human 3 (MSH3) gene by promoter methylation in nasopharyngeal carcinoma (NPC). 
      Methylation‑specific PCR, semi‑quantitative reverse transcription PCR and 
      immunohistochemical analysis were used to detect methylation and the mRNA and 
      protein expression levels of MSH3 in 54 cases of NPC tissues and 16 cases of 
      normal nasopharyngeal epithelial (NNE) tissues. The association between promoter 
      methylation and mRNA expression, and the mRNA and protein expression of the gene 
      and clinical factors was analyzed. The promoter methylation of MSH3 was detected 
      in 50% (27/54) of the primary tumors, but not in the 16 NNE tissues. The mRNA and 
      protein expression levels were significantly decreased in the 54 cases of human 
      NPC as compared to the 16 NNE tissues (P<0.05). The MSH3‑methylated cases 
      exhibited significantly lower mRNA and protein expression levels than the 
      unmethylated cases (P<0.05). The MSH3 mRNA and protein expression levels were 
      significantly associated with the variable T stage (P<0.05); however, they did 
      not correlate with the age and sex of the patients, or with the N stage, TNM 
      classification or histopathological subtype (P>0.05). On the whole, MSH3 was 
      frequently inactivated by promoter methylation and its mRNA and protein 
      expression correlated with the primary tumor stage in NPC.
FAU - Ni, Haifeng
AU  - Ni H
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Jiang, Bo
AU  - Jiang B
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Zhou, Zhen
AU  - Zhou Z
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Yuan, Xiaoyang
AU  - Yuan X
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Cao, Xiaolin
AU  - Cao X
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Huang, Guangwu
AU  - Huang G
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Li, Yong
AU  - Li Y
AD  - Department of Otolaryngology, Affiliated Hangzhou First People's Hospital of 
      Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170627
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (DNA, Neoplasm)
RN  - 0 (MSH3 protein, human)
RN  - 0 (MutS Homolog 3 Protein)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Carcinoma/genetics/*metabolism/pathology
MH  - *DNA Methylation
MH  - DNA, Neoplasm/genetics/*metabolism
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MutS Homolog 3 Protein/*biosynthesis/genetics
MH  - Nasopharyngeal Carcinoma
MH  - Nasopharyngeal Neoplasms/genetics/*metabolism/pathology
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - Neoplasm Staging
MH  - *Promoter Regions, Genetic
PMC - PMC5547962
EDAT- 2017/06/29 06:00
MHDA- 2018/04/18 06:00
PMCR- 2017/06/27
CRDT- 2017/06/29 06:00
PHST- 2015/12/07 00:00 [received]
PHST- 2017/06/22 00:00 [accepted]
PHST- 2017/06/29 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
PHST- 2017/06/29 06:00 [entrez]
PHST- 2017/06/27 00:00 [pmc-release]
AID - ijmm-40-03-0673 [pii]
AID - 10.3892/ijmm.2017.3044 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Sep;40(3):673-678. doi: 10.3892/ijmm.2017.3044. Epub 2017 Jun 
      27.