PMID- 28658176
OWN - NLM
STAT- MEDLINE
DCOM- 20170717
LR  - 20210109
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 96
IP  - 26
DP  - 2017 Jun
TI  - Circulating fibrocytes are involved in inflammation and leukocyte trafficking in 
      neonates with necrotizing enterocolitis.
PG  - e7400
LID - 10.1097/MD.0000000000007400 [doi]
LID - e7400
AB  - Fibrocytes, ahematopoietic stem cell source of fibroblasts/myofibroblasts, were 
      previously implicated to infiltrate into the intestinal and enhance 
      inflammation.The aims of the present study were to elucidate the role of 
      fibrocytes in necrotizing enterocolitis (NEC) pathogenesis and to explore the 
      mechanisms by which fibrocytes contributed to the inflammatory responses.We 
      investigated circulating and intestinal local fibrocytes from 32 patients with 
      NEC, 8 patients with noninflammatory conditions of the gastrointestinal tract and 
      12 normal subjects.Significantly higher numbers of circulating fibrocytes were 
      found in the peripheral blood from NEC patients than the controls (P < .01). 
      Numerous fibrocytes were found infiltrating the NEC intestinal mucous membranes. 
      The percentage of fibrocytes to total leukocytes in the NEC inflammatory lesions 
      was significantly increased compared with the percentage in the noninflammatory 
      gastrointestinal tract. The fibrocyte attractant chemokine C-X-C motif chemokine 
      ligand 12 (CXCL12) was significantly increased in the plasma and was detectable 
      in 80% of the peritoneal lavage fluid from NEC patients but not the controls. 
      Furthermore, chemokine expression was increased in fibrocytes infiltrating and 
      trafficking to leukocyte sites. In culture, lipopolysaccharide (LPS) induced a 
      significant increase in the expression of the Toll-like receptor (TLR4) signal, 
      with the upregulation of p38 in both the isolated fibrocytes and macrophages. 
      Similarly, interleukin (IL)-1beta induced increased the upregulation of the IL-6, 
      tumor necrosis factor (TNF)-alpha, and intercellular cell adhesion molecule-1 mRNAs 
      but downregulated ColI in fibrocytes isolated from NEC patients compared with the 
      controls.These findings indicate that circulating fibrocytes are increased in NEC 
      patients and may be recruited to the inflammatory intestinal track, most likely 
      through the CXCR4/CXCL12 axis. These cells may contribute to intestinal 
      inflammation through TLR4 signaling by producing the TNF-alpha and IL-6 cytokines.
FAU - Liu, Ye
AU  - Liu Y
AD  - Department of Neonatal, Children's Hospital, Chongqing Medical University, 
      Chongqing Department of Pathology, Linyi People's Hospital, Linyi, Shandong 
      Province Department of Pediatric General Surgery Ministry of Education Key 
      Laboratory of Child Development and Disorders, Children's Hospital, Chongqing 
      Medical University Department of Neonatology, Yongchuan Hospital, Chongqing 
      Medical University, Chongqing, P.R. China.
FAU - Qingjuan, Shang
AU  - Qingjuan S
FAU - Gao, Zongwei
AU  - Gao Z
FAU - Deng, Chun
AU  - Deng C
FAU - Wang, Yan
AU  - Wang Y
FAU - Guo, Chunbao
AU  - Guo C
LA  - eng
PT  - Journal Article
PT  - Observational Study
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (Biomarkers)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (ICAM1 protein, human)
RN  - 0 (IL1B protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Biomarkers/blood
MH  - Cell Count
MH  - Cell Movement/physiology
MH  - Cells, Cultured
MH  - Chemokine CXCL12/blood
MH  - Enterocolitis, Necrotizing/*blood/*immunology/pathology/surgery
MH  - Humans
MH  - Infant, Newborn
MH  - Intercellular Adhesion Molecule-1/blood
MH  - Interleukin-1beta/blood
MH  - Interleukin-6/blood
MH  - Leukocytes/*immunology/pathology
MH  - Lipopolysaccharides
MH  - RNA, Messenger/blood
MH  - Toll-Like Receptor 4/blood
MH  - Tumor Necrosis Factor-alpha/blood
MH  - p38 Mitogen-Activated Protein Kinases/blood
PMC - PMC5500098
EDAT- 2017/06/29 06:00
MHDA- 2017/07/18 06:00
PMCR- 2017/06/30
CRDT- 2017/06/29 06:00
PHST- 2017/06/29 06:00 [entrez]
PHST- 2017/06/29 06:00 [pubmed]
PHST- 2017/07/18 06:00 [medline]
PHST- 2017/06/30 00:00 [pmc-release]
AID - 00005792-201706300-00084 [pii]
AID - MD-D-17-01945 [pii]
AID - 10.1097/MD.0000000000007400 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2017 Jun;96(26):e7400. doi: 10.1097/MD.0000000000007400.