PMID- 28659738
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1654-661X (Print)
IS  - 1654-661X (Electronic)
IS  - 1654-661X (Linking)
VI  - 61
IP  - 1
DP  - 2017
TI  - Rosmarinic acid suppresses adipogenesis, lipolysis in 3T3-L1 adipocytes, 
      lipopolysaccharide-stimulated tumor necrosis factor-alpha secretion in macrophages, 
      and inflammatory mediators in 3T3-L1 adipocytes.
PG  - 1330096
LID - 10.1080/16546628.2017.1330096 [doi]
LID - 1330096
AB  - Background: Rosmarinic acid (RA) is a natural phenol carboxylic acid with many 
      promising biological effects. It may be a suitable candidate for improving 
      obesity-related adipose tissue dysfunction. Objective: We aimed to investigate 
      the therapeutic use of RA as an anti-obesity agent by measuring its effects on 
      adipogenesis, lipolysis, and messenger RNA (mRNA) expression of major adipokines 
      in 3T3-L1 adipocytes; and its effects on lipopolysaccharide (LPS)-induced tumor 
      necrosis factor-alpha (TNF-alpha) secretion in macrophages and inflammatory mediators in 
      3T3-L1 adipocytes incubated with macrophage-conditioned medium (MCM). Methods: 
      3T3-L1 preadipocytes were used to explore how RA affects adipogenesis, as well as 
      the involvement of phosphorylated extracellular signal-regulated kinase-1/2 
      (p-ERK1/2) and mothers against decapentaplegic homolog 3 (p-Smad3). 3T3-L1 
      preadipocytes were also differentiated into mature adipocytes to explore how RA 
      affects basal and isoproterenol- and forskolin-stimulated lipolysis; and how RA 
      affects key adipokines' mRNA expression. RAW 264.7 macrophages were stimulated 
      with LPS in the absence or presence of RA to explore RA's effects on TNF-alpha 
      secretion. MCM was collected and 3T3-L1 adipocytes were incubated with MCM to 
      explore RA's effects on interleukin-6 (IL-6), IL-1beta, monocyte chemoattractant 
      protein-1 (MCP-1), and RANTES mRNA expression. Results: During the preadipocyte 
      differentiation process, RA suppressed peroxisome proliferator-activated 
      receptor-gamma and CCAAT/enhancer binding protein-alpha, and activated p-ERK1/2 and 
      p-Smad3; inhibition of adipogenesis by RA was partially restored following 
      treatment with p-ERK1/2 and p-Smad3 inhibitors. In mature adipocytes, RA 
      inhibited basal lipolysis; phosphodiesterase-3 inhibitor reversed this. RA also 
      inhibited isoproterenol- and forskolin-stimulated glycerol and free fatty acid 
      release, and the phosphorylation of hormone-sensitive lipase and perilipin. RA 
      had no effects on leptin, adiponectin, resistin, or visfatin mRNA expression. RA 
      suppressed TNF-alpha mRNA expression and secretion in LPS-stimulated RAW 264.7 
      macrophages; and reduced LPS-MCM-induced IL-6, IL-1beta, MCP-1, and RANTES mRNA 
      expression in 3T3-L1 adipocytes. Conclusions: RA exerts inhibitory effects on 
      adipogenesis, lipolysis, and inflammation. RA could be a promising natural 
      product for improving adipose mobilization in obesity.
FAU - Rui, Yehua
AU  - Rui Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Soochow 
      University, Suzhou, PR China.
FAU - Tong, Lingxia
AU  - Tong L
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital of 
      Soochow University, Suzhou, PR China.
FAU - Cheng, Jinbo
AU  - Cheng J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Soochow 
      University, Suzhou, PR China.
FAU - Wang, Guiping
AU  - Wang G
AD  - Laboratory Animal Center, Soochow University, Suzhou, PR China.
FAU - Qin, Liqiang
AU  - Qin L
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Soochow 
      University, Suzhou, PR China.
FAU - Wan, Zhongxiao
AU  - Wan Z
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Soochow 
      University, Suzhou, PR China.
AD  - Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric 
      Disease, Soochow University, Suzhou, PR China.
LA  - eng
PT  - Journal Article
DEP - 20170607
PL  - Sweden
TA  - Food Nutr Res
JT  - Food & nutrition research
JID - 101488795
PMC - PMC5475298
OTO - NOTNLM
OT  - Rosmarinic acid
OT  - adipocytes
OT  - adipogenesis
OT  - adipokines
OT  - lipolysis
EDAT- 2017/07/01 06:00
MHDA- 2017/07/01 06:01
PMCR- 2017/06/07
CRDT- 2017/06/30 06:00
PHST- 2016/12/21 00:00 [received]
PHST- 2017/05/05 00:00 [accepted]
PHST- 2017/06/30 06:00 [entrez]
PHST- 2017/07/01 06:00 [pubmed]
PHST- 2017/07/01 06:01 [medline]
PHST- 2017/06/07 00:00 [pmc-release]
AID - 1330096 [pii]
AID - 10.1080/16546628.2017.1330096 [doi]
PST - epublish
SO  - Food Nutr Res. 2017 Jun 7;61(1):1330096. doi: 10.1080/16546628.2017.1330096. 
      eCollection 2017.