PMID- 28659882
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230804
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Dendritic Cells Primed with Paracoccidioides brasiliensis Peptide P10 Are 
      Therapeutic in Immunosuppressed Mice with Paracoccidioidomycosis.
PG  - 1057
LID - 10.3389/fmicb.2017.01057 [doi]
LID - 1057
AB  - Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, 
      with the highest prevalence in Brazil, Colombia, and Venezuela. Fungi of the 
      Paracoccidioides genus are etiologic agents of the disease. The 15 amino acid 
      peptide P10 is derived from gp43, the main diagnostic antigen of Paracoccidioides 
      brasiliensis. We previously reported that P10-pulsed dendritic cells (DCs) induce 
      a protective response against P. brasiliensis. Presently, dexamethasone-treated 
      BALB/c mice were intratracheally infected with P. brasiliensis Pb18 to establish 
      the therapeutic efficacy of P10-pulsed DCs. Immunosuppressed and infected animals 
      that received DCs had a reduction in their fungal burden, and this result was 
      most pronounced in mice receiving DCs primed with P10. The efficacy of 
      therapeutic DCs was significantly augmented by concomitant treatment with 
      trimethoprim-sulfamethoxazole. Additionally, primed-DCs with or without the 
      antifungal drug induced a beneficial Th(1)-biased immune response and 
      significantly reduced tissue damage. In conclusion, these studies with 
      immunocompromised mice demonstrate that P10-pulsed DCs with or without 
      concomitant antifungal drugs are potently effective in combating invasive PCM. 
      These findings support further translational studies to validate the use of 
      P10-primed DCs for PCM in immunocompetent and immunosuppressed hosts.
FAU - Silva, Leandro B R
AU  - Silva LBR
AD  - Laboratory of Medical Mycology, Tropical Medicine Institute USP-LIM53, University 
      of Sao PauloSao Paulo, Brazil.
FAU - Dias, Lucas S
AU  - Dias LS
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      PauloSao Paulo, Brazil.
FAU - Rittner, Glauce M G
AU  - Rittner GMG
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      PauloSao Paulo, Brazil.
FAU - Munoz, Julian E
AU  - Munoz JE
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      PauloSao Paulo, Brazil.
FAU - Souza, Ana C O
AU  - Souza ACO
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      PauloSao Paulo, Brazil.
FAU - Nosanchuk, Joshua D
AU  - Nosanchuk JD
AD  - Department of Medicine, Albert Einstein College of Medicine, BronxNY, United 
      States.
AD  - Department of Microbiology and Immunology, Albert Einstein College of Medicine, 
      BronxNY, United States.
FAU - Travassos, Luiz R
AU  - Travassos LR
AD  - Department of Microbiology, Immunology and Parasitology, Federal University of 
      Sao PauloSao Paulo, Brazil.
FAU - Taborda, Carlos P
AU  - Taborda CP
AD  - Laboratory of Medical Mycology, Tropical Medicine Institute USP-LIM53, University 
      of Sao PauloSao Paulo, Brazil.
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      PauloSao Paulo, Brazil.
LA  - eng
GR  - R01 AI052733/AI/NIAID NIH HHS/United States
GR  - R21 AI124797/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20170614
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5469887
OTO - NOTNLM
OT  - P10
OT  - Paracoccidioides brasiliensis
OT  - adjuvants
OT  - dendritic cells
OT  - paracoccidioidomycosis
OT  - vaccine
EDAT- 2017/07/01 06:00
MHDA- 2017/07/01 06:01
PMCR- 2017/06/14
CRDT- 2017/06/30 06:00
PHST- 2016/10/10 00:00 [received]
PHST- 2017/05/26 00:00 [accepted]
PHST- 2017/06/30 06:00 [entrez]
PHST- 2017/07/01 06:00 [pubmed]
PHST- 2017/07/01 06:01 [medline]
PHST- 2017/06/14 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.01057 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Jun 14;8:1057. doi: 10.3389/fmicb.2017.01057. eCollection 
      2017.