PMID- 28660578
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180625
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1609
DP  - 2017
TI  - Combined Use of MALDI-TOF Mass Spectrometry and (31)P NMR Spectroscopy for 
      Analysis of Phospholipids.
PG  - 107-122
LID - 10.1007/978-1-4939-6996-8_11 [doi]
AB  - Lipids are important and abundant constituents of all biological tissues and body 
      fluids. In particular, phospholipids (PL) constitute a major part of the cellular 
      membrane, play a role in signal transduction, and some selected PL are 
      increasingly considered as potential disease markers. However, methods of lipid 
      analysis are less established in comparison to techniques of protein analysis. 
      Mass spectrometry (MS) is an increasingly used technique to analyze lipids, 
      especially in combination with electrospray ionization (ESI) MS which is the so 
      far best established ionization method. Matrix-assisted laser 
      desorption/ionization time-of-flight (MALDI-TOF) MS has itself proven to be also 
      useful in the field of lipid analysis. (31)P nuclear magnetic resonance (NMR) 
      spectroscopy is another powerful method of PL analysis, represents a direct 
      quantitative method, and does not suffer from suppression effects.This chapter 
      gives an overview of methodological aspects of MALDI-TOF MS and (31)P NMR in 
      lipid research and summarizes the specific advantages and drawbacks of both 
      methods. In particular, suppression effects in MS will be highlighted and 
      possible ways to overcome this problem (use of different matrices, separation of 
      the relevant lipid mixture prior to analysis) will be discussed.
FAU - Schroter, Jenny
AU  - Schroter J
AD  - Institute for Medical Physics and Biophysics, University of Leipzig, Leipzig 
      04107, Germany.
FAU - Popkova, Yulia
AU  - Popkova Y
AD  - Institute for Medical Physics and Biophysics, University of Leipzig, Leipzig 
      04107, Germany.
FAU - Suss, Rosmarie
AU  - Suss R
AD  - Institute for Medical Physics and Biophysics, University of Leipzig, Leipzig 
      04107, Germany.
FAU - Schiller, Jurgen
AU  - Schiller J
AD  - Institute for Medical Physics and Biophysics, University of Leipzig, Leipzig 
      04107, Germany. juergen.schiller@medizin.uni-leipzig.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Phospholipids)
SB  - IM
MH  - Humans
MH  - Metabolome
MH  - Metabolomics/methods
MH  - *Nuclear Magnetic Resonance, Biomolecular/methods
MH  - Phospholipids/*analysis/*chemistry
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
OTO - NOTNLM
OT  - 31P NMR spectroscopy
OT  - Glycerophospholipids
OT  - Lipid analysis
OT  - Lipid extracts
OT  - MALDI-TOF MS
OT  - Matrix
EDAT- 2017/07/01 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/06/30 06:00
PHST- 2017/06/30 06:00 [entrez]
PHST- 2017/07/01 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
AID - 10.1007/978-1-4939-6996-8_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1609:107-122. doi: 10.1007/978-1-4939-6996-8_11.