PMID- 2866188
OWN - NLM
STAT- MEDLINE
DCOM- 19860116
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 260
IP  - 29
DP  - 1985 Dec 15
TI  - Transit of receptors for epidermal growth factor and transferrin through 
      clathrin-coated pits. Analysis of the kinetics of receptor entry.
PG  - 15938-45
AB  - Selective enrichment of clathrin-coated membranes by anticlathrin 
      immunoadsorption was used to examine the internalization of receptor-ligand 
      complexes through coated pits. Using Staphylococcus aureus-anticlathrin antibody 
      and [35S]methionine-labeled KB cells, the kinetics of association of the 
      epidermal growth factor (EGF-R) and transferrin receptors (TF-R) with coated 
      membranes were directly examined. The accumulation of EGF-R in coated pits at the 
      cell surface was dependent upon EGF binding. EGF-R then passed sequentially 
      through a compartment which did not react with anticlathrin antibody and a second 
      clathrin-coated compartment. The EGF-R was degraded in lysosomes with a half-life 
      of approximately 41-55 min. The tumor promoter, 4 beta-phorbol 12-myristate 
      13-acetate, appears to mimic the action of EGF in inducing EGF-R accumulation in 
      coated pits at the cell surface and receptor internalization. In contrast to the 
      results with EGF-R, the TF-R was found in clathrin-coated membranes in the 
      presence or absence of TF, and the concentration of TF-R in clathrin-coated 
      membranes did not significantly change with time. The method presented should be 
      of great utility for examining the biochemical changes that occur during the 
      receptor-mediated endocytosis and sorting of ligands and receptors.
FAU - Hanover, J A
AU  - Hanover JA
FAU - Beguinot, L
AU  - Beguinot L
FAU - Willingham, M C
AU  - Willingham MC
FAU - Pastan, I H
AU  - Pastan IH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Clathrin)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Transferrin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active
MH  - Clathrin
MH  - Coated Pits, Cell-Membrane/*metabolism
MH  - Endosomes/*metabolism
MH  - Epidermal Growth Factor/metabolism
MH  - ErbB Receptors
MH  - Half-Life
MH  - Humans
MH  - Immunosorbent Techniques
MH  - Kinetics
MH  - Rabbits
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Transferrin
MH  - Surface Properties
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1985/12/15 00:00
MHDA- 1985/12/15 00:01
CRDT- 1985/12/15 00:00
PHST- 1985/12/15 00:00 [pubmed]
PHST- 1985/12/15 00:01 [medline]
PHST- 1985/12/15 00:00 [entrez]
AID - S0021-9258(17)36349-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1985 Dec 15;260(29):15938-45.