PMID- 28666092 OWN - NLM STAT- MEDLINE DCOM- 20180417 LR - 20220318 IS - 1480-3321 (Electronic) IS - 0831-2796 (Linking) VI - 60 IP - 8 DP - 2017 Aug TI - Characterization of genome in tetraploid StY species of Elymus (Triticeae: Poaceae) using sequential FISH and GISH. PG - 679-685 LID - 10.1139/gen-2017-0046 [doi] AB - Genomes of ten species of Elymus, either presumed or known as tetraploid StY, were characterized using fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). These tetraploid species could be grouped into three categories. Type I included StY genome reported species-Roegneria pendulina, R. nutans, R. glaberrima, R. ciliaris, and Elymus nevskii, and StY genome presumed species-R. sinica, R. breviglumis, and R. dura, whose genome could be separated into two sets based on different GISH intensities. Type I genome constitution was deemed as putative StY. The St genome were mainly characterized with intense hybridization with pAs1, fewer AAG sites, and linked distribution of 5S rDNA and 18S-26S rDNA, while the Y genome with less intense hybridization with pAs1, more varied AAG sites, and isolated distribution of 5S rDNA and 18S-26S rDNA. Nevertheless, further genomic variations were detected among the different StY species. Type II included E. alashanicus, whose genome could be easily separated based on GISH pattern. FISH and GISH patterns suggested that E. alashanicus comprised a modified St genome and an unknown genome. Type III included E. longearistatus, whose genome could not be separated by GISH and was designated as St(l)Y(l). Notably, a close relationship between S(l) and Y(l) genomes was observed. FAU - Liu, Ruijuan AU - Liu R AD - a Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. AD - b University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Wang, Richard R-C AU - Wang RR AD - c U.S. Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT 84322-6300, USA. FAU - Yu, Feng AU - Yu F AD - a Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. AD - b University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Lu, Xingwang AU - Lu X AD - a Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. AD - b University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Dou, Quanwen AU - Dou Q AD - a Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. AD - d Key Laboratory of Crop Molecular Breeding, Qinghai Province, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. LA - eng PT - Journal Article DEP - 20170630 PL - Canada TA - Genome JT - Genome JID - 8704544 SB - IM MH - Chromosomes, Plant MH - Elymus/*genetics MH - *Genome, Plant MH - In Situ Hybridization/*methods MH - *In Situ Hybridization, Fluorescence MH - Repetitive Sequences, Nucleic Acid MH - *Tetraploidy OTO - NOTNLM OT - Elymus species OT - St genome OT - Y genome OT - differenciation des genomes OT - especes du genre Elymus OT - genome differentiation OT - genome St OT - genome Y EDAT- 2017/07/01 06:00 MHDA- 2018/04/18 06:00 CRDT- 2017/07/01 06:00 PHST- 2017/07/01 06:00 [pubmed] PHST- 2018/04/18 06:00 [medline] PHST- 2017/07/01 06:00 [entrez] AID - 10.1139/gen-2017-0046 [doi] PST - ppublish SO - Genome. 2017 Aug;60(8):679-685. doi: 10.1139/gen-2017-0046. Epub 2017 Jun 30.