PMID- 28667931 OWN - NLM STAT- MEDLINE DCOM- 20171010 LR - 20171010 IS - 1873-2682 (Electronic) IS - 1011-1344 (Linking) VI - 173 DP - 2017 Aug TI - The impact of high-dose narrowband ultraviolet A1 on dermal thickness, collagen and matrix-metalloproteinases in animal model of scleroderma. PG - 448-455 LID - S1011-1344(17)30443-8 [pii] LID - 10.1016/j.jphotobiol.2017.06.021 [doi] AB - OBJECTIVE: The main purpose of the present study was to define the impact of high-dose of 365+/-5nm ultraviolet A1 (UVA1) on dermal fibrosis in the pre-established, bleomycin-induced mouse model of scleroderma. METHODS: DBA/2 strain mice with the pre-established, bleomycin-induced scleroderma were irradiated with cumulative UVA1 dose of 1200J/cm(2) and in parallel were challenged with prolonged administration of bleomycin. Non-treated groups served as the control. Light source emitting a narrow band UVA1 light of 365+/-5nm and 21mW/cm(2) power density was used in the study. Histological analysis was performed for the evaluation of dermal thickness. The expressions of matrix-metalloproteinase-1 (MMP-1), matrix-metalloproteinase-3 (MMP-3), collagen types I and III were evaluated by immunohistochemical analyses. The Mann - Whitney U test was used for statistical analysis. RESULTS: Dermal thickness in mice injected with bleomycin during all the experiment (8weeks) and irradiated with UVA1 for the last 5weeks was significantly lower than that in mice challenged only with bleomycin for 8weeks (253.96+/-31.83mum and 497.43+/-57.83mum, respectively; P=0.002). The dermal thickness after phototherapy was lower as compared with the pre-existing fibrotic changes observed after 3weeks of bleomycin injections (253.96+/-31.83mum and 443.87+/-41.76mum, respectively; P=0.002). High-dose of UVA1 induced the 5.8- and 5.2-fold increase in MMP-1 and MMP-3 expressions, respectively, and the 1.2- and 1.4-fold decrease in collagen type I and collagen type III expressions in the pre-established, bleomycin-induced scleroderma model as compared to that in the control non-irradiated mice (P=0.002). CONCLUSIONS: Our study has demonstrated that a cumulative 365+/-5nm UVA1 radiation dosage of 1200J/cm(2) not only prevents the progression of dermal fibrosis, but also induces a regression of pre-existing fibrotic changes. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Karpec, Diana AU - Karpec D AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania; Vilnius University, Faculty of Medicine, Center of Rheumatology, Santariskiu St. 2, LT-08661 Vilnius, Lithuania. Electronic address: diana.karpec@santa.lt. FAU - Rudys, Romualdas AU - Rudys R AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania. Electronic address: romualdas.rudys@imcentras.lt. FAU - Leonaviciene, Laima AU - Leonaviciene L AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania. Electronic address: laima.leonaviciene@imcentras.lt. FAU - Mackiewicz, Zygmunt AU - Mackiewicz Z AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania. Electronic address: zygmunt.mackiewicz@imcentras.lt. FAU - Bradunaite, Ruta AU - Bradunaite R AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania. Electronic address: ruta.bradunaite@imcentras.lt. FAU - Kirdaite, Gailute AU - Kirdaite G AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania. Electronic address: gailute.kirdaite@imcentras.lt. FAU - Venalis, Algirdas AU - Venalis A AD - State Research Institute Centre for Innovative Medicine, Santariskiu St. 5, LT-08406 Vilnius, Lithuania; Vilnius University, Faculty of Medicine, Center of Rheumatology, Santariskiu St. 2, LT-08661 Vilnius, Lithuania. Electronic address: algirdas.venalis@imcentras.lt. LA - eng PT - Journal Article DEP - 20170621 PL - Switzerland TA - J Photochem Photobiol B JT - Journal of photochemistry and photobiology. B, Biology JID - 8804966 RN - 0 (Collagen Type I) RN - 0 (Collagen Type III) RN - 11056-06-7 (Bleomycin) RN - 9007-34-5 (Collagen) RN - EC 3.4.24.- (Matrix Metalloproteinases) RN - EC 3.4.24.17 (Matrix Metalloproteinase 3) RN - EC 3.4.24.7 (Matrix Metalloproteinase 1) SB - IM MH - Animals MH - Bleomycin/toxicity MH - Collagen/*metabolism MH - Collagen Type I/metabolism MH - Collagen Type III/metabolism MH - Dermis/physiology/*radiation effects MH - Disease Models, Animal MH - Female MH - Immunohistochemistry MH - Matrix Metalloproteinase 1/metabolism MH - Matrix Metalloproteinase 3/metabolism MH - Matrix Metalloproteinases/*metabolism MH - Mice MH - Mice, Inbred DBA MH - Scleroderma, Localized/chemically induced/*radiotherapy MH - Skinfold Thickness MH - *Ultraviolet Rays MH - Ultraviolet Therapy OTO - NOTNLM OT - Animal model OT - Phototherapy OT - Scleroderma OT - Ultraviolet A1 EDAT- 2017/07/02 06:00 MHDA- 2017/10/11 06:00 CRDT- 2017/07/02 06:00 PHST- 2017/03/31 00:00 [received] PHST- 2017/06/13 00:00 [revised] PHST- 2017/06/20 00:00 [accepted] PHST- 2017/07/02 06:00 [pubmed] PHST- 2017/10/11 06:00 [medline] PHST- 2017/07/02 06:00 [entrez] AID - S1011-1344(17)30443-8 [pii] AID - 10.1016/j.jphotobiol.2017.06.021 [doi] PST - ppublish SO - J Photochem Photobiol B. 2017 Aug;173:448-455. doi: 10.1016/j.jphotobiol.2017.06.021. Epub 2017 Jun 21.