PMID- 28668766
OWN - NLM
STAT- MEDLINE
DCOM- 20180627
LR  - 20180627
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 82
DP  - 2017 Oct
TI  - Effect of arginine on the growth and biofilm formation of oral bacteria.
PG  - 256-262
LID - S0003-9969(17)30203-0 [pii]
LID - 10.1016/j.archoralbio.2017.06.026 [doi]
AB  - BACKGROUND: Alkali production via arginine deiminase system (ADS) of oral 
      bacteria plays a significant role in oral ecology, pH homeostasis and inhibition 
      of dental caries. ADS activity in dental plaque varies greatly between 
      individuals, which may profoundly affect their susceptibility to caries. 
      OBJECTIVE: To investigate the effect of arginine on the growth and biofilm 
      formation of oral bacteria. METHODS AND RESULTS: Polymicrobial dental biofilms 
      derived from saliva were formed in a high-throughput active attachment biofilm 
      model and l-arginine (Arg) was shown to reduce the colony forming units (CFU) 
      counts of such biofilms grown for various periods or biofilms derived from saliva 
      of subjects with different caries status. Arg hardly disturbed bacterial growth 
      of Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis and 
      Streptococcus gordonii in BHI medium, but only inhibited biofilm formation of S. 
      mutans. Scanning electron microscope (SEM) showed S. mutans biofilms harboured 
      fewer cells grown with Arg than that without Arg, even in the initial 2h and 8h 
      phase. Confocal laser scanning microscope (CLSM) images of poly-microbial dental 
      and S. mutans biofilms revealed the biofilms grown with Arg had lower 
      exopolysaccharide (EPS)/bacteria ratios than those without Arg (P=0.004, 0.002, 
      respectively). Arg could significantly reduce the production of water-insoluble 
      EPS in S. mutans biofilms (P<0.001); however, quantitative real-time PCR 
      (qRT-PCR) did not show significantly influence in gene expression of gtfB, gtfC 
      or gtfD (P=0.32, 0.06, 0.44 respectively). CONCLUSIONS: Arg could reduce the 
      biomass of poly-microbial dental biofilms and S. mutans biofilms, which may be 
      due to the impact of Arg on water-insoluble EPS. Considering the contribution to 
      pH homeostasis in dental biofilms, Arg may serve as an important agent keeping 
      oral biofilms healthy thus prevent dental caries.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Huang, Xuelian
AU  - Huang X
AD  - Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam 
      (ACTA), University of Amsterdam and Free University Amsterdam, Amsterdam, The 
      Netherlands; State Key Laboratory of Oral Diseases, West China College of 
      Stomatology, Sichuan University, Chengdu, Sichuan, China; Division of General 
      Dentistry, Eastman Institute for Oral Health, University of Rochester, Rochester, 
      NY, USA.
FAU - Zhang, Keke
AU  - Zhang K
AD  - State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China; School and Hospital of Stomatology, Wenzhou 
      Medical University, Wenzhou, Zhejiang, China.
FAU - Deng, Meng
AU  - Deng M
AD  - State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China; Oral Biology Program, School of Dentistry, 
      University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
FAU - Exterkate, Robertus A M
AU  - Exterkate RAM
AD  - Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam 
      (ACTA), University of Amsterdam and Free University Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Liu, Chengcheng
AU  - Liu C
AD  - State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Zhou, Xuedong
AU  - Zhou X
AD  - State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Cheng, Lei
AU  - Cheng L
AD  - State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Ten Cate, Jacob M
AU  - Ten Cate JM
AD  - Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam 
      (ACTA), University of Amsterdam and Free University Amsterdam, Amsterdam, The 
      Netherlands. Electronic address: j.t.cate@acta.nl.
LA  - eng
PT  - Journal Article
DEP - 20170624
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Arginine/*pharmacology
MH  - Biofilms/*drug effects/*growth & development
MH  - Dental Caries/microbiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Real-Time Polymerase Chain Reaction
MH  - Saliva/*microbiology
MH  - Stem Cells
MH  - Streptococcus gordonii/drug effects
MH  - Streptococcus mutans/*drug effects
MH  - Streptococcus sanguis/drug effects
MH  - Streptococcus sobrinus/drug effects
OTO - NOTNLM
OT  - Alkali
OT  - Biofilms
OT  - Dental caries
OT  - Exopolysaccharide
OT  - Microbial viability
OT  - Streptococcus
OT  - gtf
EDAT- 2017/07/03 06:00
MHDA- 2018/06/28 06:00
CRDT- 2017/07/03 06:00
PHST- 2017/04/26 00:00 [received]
PHST- 2017/06/19 00:00 [revised]
PHST- 2017/06/20 00:00 [accepted]
PHST- 2017/07/03 06:00 [pubmed]
PHST- 2018/06/28 06:00 [medline]
PHST- 2017/07/03 06:00 [entrez]
AID - S0003-9969(17)30203-0 [pii]
AID - 10.1016/j.archoralbio.2017.06.026 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2017 Oct;82:256-262. doi: 10.1016/j.archoralbio.2017.06.026. Epub 
      2017 Jun 24.