PMID- 28669017
OWN - NLM
STAT- MEDLINE
DCOM- 20180612
LR  - 20181113
IS  - 1435-4373 (Electronic)
IS  - 0934-9723 (Linking)
VI  - 36
IP  - 11
DP  - 2017 Nov
TI  - Bartonella henselae is usually not viable in lymph nodes of patients with cat 
      scratch disease.
PG  - 2207-2213
LID - 10.1007/s10096-017-3047-z [doi]
AB  - Bartonella henselae, the agent of cat scratch disease (CSD), appears to be a 
      common organism responsible for lymphadenitis in both adults and children. There 
      is a very low isolation rate for B. henselae from lymph nodes of patients with 
      CSD. Our objective was to evaluate B. henselae viability in a large series of 
      lymph nodes from patients with CSD. From January to November 2016, we analyzed 
      lymph node biopsy samples from patients diagnosed with CSD. We used reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR) to detect B. 
      henselae RNA, as well as cultures, histological analyses, and fluorescence in 
      situ hybridization (FISH). We tested 87 lymph nodes positive for B. henselae DNA 
      but only 8 (9%) presented with B. henselae RNA. We did not find a significant 
      difference for the pap threshold cycle (C(T)) values between RNA-positive and 
      RNA-negative lymph nodes (p = 0.5). Cultures, histological analyses, and FISH 
      were negative for all the tested samples. We provide evidence that B. henselae 
      are not or are rarely viable in most cases in the lymph nodes of patients with 
      CSD.
FAU - Prudent, E
AU  - Prudent E
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Lepidi, H
AU  - Lepidi H
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Audoly, G
AU  - Audoly G
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - La Scola, B
AU  - La Scola B
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Fournier, P-E
AU  - Fournier PE
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Edouard, S
AU  - Edouard S
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Angelakis, E
AU  - Angelakis E
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France.
FAU - Raoult, D
AU  - Raoult D
AD  - URMITE, Aix Marseille Universite, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - 
      Mediterranee Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, 
      France. Didier.raoult@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20170702
PL  - Germany
TA  - Eur J Clin Microbiol Infect Dis
JT  - European journal of clinical microbiology & infectious diseases : official 
      publication of the European Society of Clinical Microbiology
JID - 8804297
RN  - 0 (RNA, Bacterial)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bartonella henselae/genetics/growth & development/*isolation & purification
MH  - Cat-Scratch Disease/*microbiology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymph Nodes/*microbiology
MH  - Lymphadenitis/*microbiology
MH  - Male
MH  - Middle Aged
MH  - RNA, Bacterial/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2017/07/03 06:00
MHDA- 2018/06/13 06:00
CRDT- 2017/07/03 06:00
PHST- 2017/03/07 00:00 [received]
PHST- 2017/06/18 00:00 [accepted]
PHST- 2017/07/03 06:00 [pubmed]
PHST- 2018/06/13 06:00 [medline]
PHST- 2017/07/03 06:00 [entrez]
AID - 10.1007/s10096-017-3047-z [pii]
AID - 10.1007/s10096-017-3047-z [doi]
PST - ppublish
SO  - Eur J Clin Microbiol Infect Dis. 2017 Nov;36(11):2207-2213. doi: 
      10.1007/s10096-017-3047-z. Epub 2017 Jul 2.