PMID- 28669387
OWN - NLM
STAT- MEDLINE
DCOM- 20171127
LR  - 20171128
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 189
DP  - 2017 Jul
TI  - Amplification and selection of PRRSV-activated VDJ repertoires in pigs secreting 
      distinct neutralizing antiboidies.
PG  - 53-57
LID - S0165-2427(16)30397-X [pii]
LID - 10.1016/j.vetimm.2017.06.005 [doi]
AB  - Neutralizing antibodies (nAbs) play an important role in protective immunity 
      against porcine reproductive and respiratory syndrome virus (PRRSV) infection. 
      However, the characterization of PRRSV nAb repertoires is rarely investigated. In 
      this study, we developed a swine VDJ amplification method and selection criteria 
      for the characterization of PRRSV-activated VDJ repertoires. According to clonal 
      expansion theory, two separated aliquots of lymph nodes from pigs producing 
      different PRRSV nAbs were utilized to determine the activated B-cell repertoires. 
      Swine VDJ repertoires from a mock-infected pig and PRRSV-infected pigs secreting 
      no detectable nAbs, only homologous nAbs, and broad nAbs were amplified by a 
      single pair of primers that could detect all seven major VDJ genes. The amplicons 
      were cloned and sequenced to generate 385 VDJ sequences. Sequence alignment 
      showed that the diversification of VDJ genes was mainly due to the variation in 
      complementarity determining regions (CDRs), especially CDR3. Based on selection 
      criteria, shared and abundant sequences were identified in two separated aliquots 
      from PRRSV-infected pigs but not from the mock-infected pig, suggesting they were 
      secreted from PRRSV-activated B cells. Thus, the amplification and selection 
      method provide a potential alternative for the characterization of swine VDJ 
      repertoires. However, additional experiments are required to determine whether 
      the shared and abundant VDJ lineages identified in this study are PRRSV-specific 
      or distinct neutralizing-antibodies-associated.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Chen, Nanhua
AU  - Chen N
AD  - College of Veterinary Medicine, Yangzhou University, Jiangsu 225009, PR China; 
      Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States. Electronic 
      address: chnhlh@126.com.
FAU - Trible, Benjamin R
AU  - Trible BR
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States.
FAU - Rowland, Raymond R R
AU  - Rowland RRR
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States.
LA  - eng
PT  - Journal Article
DEP - 20170620
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Neutralizing)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/*immunology
MH  - Antibody Formation/immunology
MH  - Cloning, Molecular
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Sequence Alignment/veterinary
MH  - Sequence Analysis, DNA/veterinary
MH  - Swine/immunology/virology
MH  - V(D)J Recombination/genetics/*immunology
OTO - NOTNLM
OT  - Complementarity determining regions (CDRs)
OT  - Neutralizing antibodies (nAbs)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - VDJ repertoires
EDAT- 2017/07/04 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/07/04 06:00
PHST- 2016/12/31 00:00 [received]
PHST- 2017/04/25 00:00 [revised]
PHST- 2017/06/19 00:00 [accepted]
PHST- 2017/07/04 06:00 [entrez]
PHST- 2017/07/04 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - S0165-2427(16)30397-X [pii]
AID - 10.1016/j.vetimm.2017.06.005 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2017 Jul;189:53-57. doi: 10.1016/j.vetimm.2017.06.005. 
      Epub 2017 Jun 20.