PMID- 28673559
OWN - NLM
STAT- MEDLINE
DCOM- 20180510
LR  - 20180510
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 44
DP  - 2017 Oct
TI  - CON4EI: Bovine Corneal Opacity and Permeability (BCOP) test for hazard 
      identification and labelling of eye irritating chemicals.
PG  - 122-133
LID - S0887-2333(17)30184-4 [pii]
LID - 10.1016/j.tiv.2017.06.028 [doi]
AB  - Assessment of ocular irritation potential is an international regulatory 
      requirement in the safety evaluation of industrial and consumer products. None in 
      vitro ocular irritation assays are capable of fully categorizing chemicals as 
      stand-alone. Therefore, the CEFIC-LRI-AIMT6-VITO CON4EI consortium assessed the 
      reliability of eight in vitro test methods and computational models as well as 
      established a tiered-testing strategy. One of the selected assays was Bovine 
      Corneal Opacity and Permeability (BCOP). In this project, the same corneas were 
      used for measurement of opacity using the OP-KIT, the Laser Light-Based 
      Opacitometer (LLBO) and for histopathological analysis. The results show that the 
      accuracy of the BCOP OP-KIT in identifying Cat 1 chemicals was 73.8% while the 
      accuracy was 86.3% for No Cat chemicals. BCOP OP-KIT false negative results were 
      often related to an in vivo classification driven by conjunctival effects only. 
      For the BCOP LLBO, the accuracy in identifying Cat 1 chemicals was 74.4% versus 
      88.8% for No Cat chemicals. The BCOP LLBO seems very promising for the 
      identification of No Cat liquids but less so for the identification of solids. 
      Histopathology as an additional endpoint to the BCOP test method does not reduce 
      the false negative rate substantially for in vivo Cat 1 chemicals.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Verstraelen, Sandra
AU  - Verstraelen S
AD  - VITO NV (Flemish Institute for Technological Research), Mol, Belgium. Electronic 
      address: sandra.verstraelen@vito.be.
FAU - Maglennon, Gareth
AU  - Maglennon G
AD  - Envigo, Cambridgeshire, United Kingdom.
FAU - Hollanders, Karen
AU  - Hollanders K
AD  - VITO NV (Flemish Institute for Technological Research), Mol, Belgium.
FAU - Boonen, Francis
AU  - Boonen F
AD  - VITO NV (Flemish Institute for Technological Research), Mol, Belgium.
FAU - Adriaens, Els
AU  - Adriaens E
AD  - Adriaens Consulting BVBA, Aalter, Belgium.
FAU - Alepee, Nathalie
AU  - Alepee N
AD  - L'Oreal Research & Innovation, Aulnay-sous-Bois, France.
FAU - Drzewiecka, Agnieszka
AU  - Drzewiecka A
AD  - Institute of Industrial Organic Chemistry Branch Pszczyna, Pszczyna, Poland.
FAU - Gruszka, Katarzyna
AU  - Gruszka K
AD  - Institute of Industrial Organic Chemistry Branch Pszczyna, Pszczyna, Poland.
FAU - Kandarova, Helena
AU  - Kandarova H
AD  - MatTek In Vitro Life Science Laboratories, Bratislava, Slovak Republic.
FAU - Willoughby, Jamin A Sr
AU  - Willoughby JA Sr
AD  - Cyprotex US, LLC, MI, USA.
FAU - Guest, Robert
AU  - Guest R
AD  - Envigo, Cambridgeshire, United Kingdom.
FAU - Schofield, Jane
AU  - Schofield J
AD  - Envigo, Cambridgeshire, United Kingdom.
FAU - Van Rompay, An R
AU  - Van Rompay AR
AD  - VITO NV (Flemish Institute for Technological Research), Mol, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20170630
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Irritants)
SB  - IM
MH  - *Animal Testing Alternatives
MH  - Animals
MH  - Cattle
MH  - Corneal Opacity/*chemically induced
MH  - Eye/*drug effects/metabolism
MH  - Irritants/*classification/*toxicity
MH  - Permeability/*drug effects
MH  - Product Labeling
OTO - NOTNLM
OT  - Bovine Corneal Opacity and Permeability (BCOP)
OT  - CON4EI
OT  - Histopathology
OT  - Laser Light-Based Opacitometer (LLBO)
OT  - OECD Test Guideline 437
OT  - OP-KIT
OT  - Ocular irritation
EDAT- 2017/07/05 06:00
MHDA- 2018/05/11 06:00
CRDT- 2017/07/05 06:00
PHST- 2017/03/27 00:00 [received]
PHST- 2017/05/30 00:00 [revised]
PHST- 2017/06/27 00:00 [accepted]
PHST- 2017/07/05 06:00 [pubmed]
PHST- 2018/05/11 06:00 [medline]
PHST- 2017/07/05 06:00 [entrez]
AID - S0887-2333(17)30184-4 [pii]
AID - 10.1016/j.tiv.2017.06.028 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2017 Oct;44:122-133. doi: 10.1016/j.tiv.2017.06.028. Epub 2017 
      Jun 30.