PMID- 28674283
OWN - NLM
STAT- MEDLINE
DCOM- 20180511
LR  - 20180511
IS  - 1348-4540 (Electronic)
IS  - 0918-8959 (Linking)
VI  - 64
IP  - 8
DP  - 2017 Aug 30
TI  - Neutral ceramidase activity inhibition is involved in palmitate-induced apoptosis 
      in INS-1 cells.
PG  - 767-776
LID - 10.1507/endocrj.EJ16-0512 [doi]
AB  - Neutral ceramidase (NCDase) is a class of ceramidases, a key enzyme in ceramide 
      degradation. Recently, it was observed that NCDase activity was suppressed by 
      saturated fatty acids to increase ceramide content in rat muscle. However, little 
      is known about its changes in activity and roles in palmitate (Palm)-induced 
      lipotoxicity in pancreatic beta cells. Here, we demonstrated that Palm treatment 
      significantly down-regulated NCDase activity, mRNA and protein levels in rat 
      INS-1 cells. In addition, Palm caused a significant accumulation of ceramide, 
      while SPH level remained unchanged, suggesting that inhibition of NCDase activity 
      led to no change of SPH level after treatment with Palm for 24 h. Furthermore, 
      NCDase overexpression significantly reduced Palm-induced apoptosis in INS-1 
      cells. Conversely, NCDase siRNA knockdown markedly exacerbated Palm-induced 
      apoptosis. In conclusion, Palm treatment suppressed the activity of NCDase and 
      down-regulated its mRNA and protein expression. Furthermore, NCDase inhibition 
      was involved in Palm-induced apoptosis by blocking ceramide degradation in INS-1 
      cells.
FAU - Luo, Fen
AU  - Luo F
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
FAU - Feng, Yamin
AU  - Feng Y
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
FAU - Ma, Huimin
AU  - Ma H
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
FAU - Liu, Chao
AU  - Liu C
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Chen, Guofang
AU  - Chen G
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Wei, Xiao
AU  - Wei X
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Mao, Xiaodong
AU  - Mao X
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Li, Xingjia
AU  - Li X
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Xu, Yijiao
AU  - Xu Y
AD  - Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of 
      Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 
      Branch of China Academy of Chinese Medical Science, Nanjing 210028, China.
FAU - Tang, Shan
AU  - Tang S
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
FAU - Wen, Honghua
AU  - Wen H
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
FAU - Jin, Junfei
AU  - Jin J
AD  - China-USA Lipids in Health and Disease Research Center, Guilin Medical 
      University, Gulin 541001, China.
FAU - Zhu, Qun
AU  - Zhu Q
AD  - Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210011, China.
LA  - eng
PT  - Journal Article
DEP - 20170701
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 3.5.1.23 (Neutral Ceramidase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Down-Regulation/drug effects
MH  - Insulin-Secreting Cells/cytology/*drug effects/enzymology
MH  - Neutral Ceramidase/*metabolism
MH  - Palmitic Acid/*pharmacology
MH  - Pancreas/cytology/*drug effects/enzymology
MH  - Rats
OTO - NOTNLM
OT  - Apoptosis
OT  - Ceramide
OT  - Neutral ceramidase
OT  - Palmitate
OT  - Pancreatic beta-cells
EDAT- 2017/07/05 06:00
MHDA- 2018/05/12 06:00
CRDT- 2017/07/05 06:00
PHST- 2017/07/05 06:00 [pubmed]
PHST- 2018/05/12 06:00 [medline]
PHST- 2017/07/05 06:00 [entrez]
AID - 10.1507/endocrj.EJ16-0512 [doi]
PST - ppublish
SO  - Endocr J. 2017 Aug 30;64(8):767-776. doi: 10.1507/endocrj.EJ16-0512. Epub 2017 
      Jul 1.