PMID- 28675985
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR  - 20210309
IS  - 1029-2470 (Electronic)
IS  - 1029-2470 (Linking)
VI  - 51
IP  - 6
DP  - 2017 Jun
TI  - Hydrogen alleviates hyperoxic acute lung injury related endoplasmic reticulum 
      stress in rats through upregulation of SIRT1.
PG  - 622-632
LID - 10.1080/10715762.2017.1351027 [doi]
AB  - Hyperoxic acute lung injury (HALI) is a major clinical problem for patients 
      undergoing supplemental oxygen therapy. Currently in clinical settings there 
      exist no effective means of prevention or treatment methods. Our previous study 
      found that: hydrogen could reduce HALI, as well as oxidative stress. This 
      research will further explore the mechanism underlying the protective effect of 
      hydrogen on oxygen toxicity. Rats were randomly assigned into three experimental 
      groups and were exposed in a oxygen chamber for 60 continuous hours: 100% 
      balanced air (control); 100% oxygen (HALI); 100% oxygen with hydrogen treatment 
      (HALI + HRS). We examined lung function by wet to dry ratio of lung, lung pleural 
      effusion and cell apoptosis. We also detected endoplasmic reticulum stress (ERS) 
      by examining the expression of CHOP, GRP78 and XBP1. We further investigated the 
      role of Sirtuin 1 (SIRT1) in HALI, which contributes to cellular regulation 
      including ERS, by examining its expression after hydrogen treatment with SIRT1 
      inhibitor. Hydrogen could significantly reduce HALI by reducing lung edema and 
      apoptosis, inhibiting the elevating of ERS and increased SIRT1 expression. By 
      inhibition of SIRT1 expression, the effect of hydrogen on prevention of HALI is 
      significantly weakened, the inhibition of the ERS was also reversed. Our findings 
      indicate that hydrogen could reduce HALI related ERS and the mechanism of 
      hydrogen may be associated with upregulation of SIRT1, this study reveals the 
      molecular mechanisms underlying the protective effect of hydrogen, which provides 
      a new theoretical basis for clinical application of hydrogen.
FAU - Sun, Qiang
AU  - Sun Q
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
FAU - Han, Wenjie
AU  - Han W
AD  - b Department of VIP Respiration Medicine , PLA Navy General Hospital , Beijing , 
      China.
FAU - Hu, Huijun
AU  - Hu H
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
FAU - Fan, Danfeng
AU  - Fan D
AD  - b Department of VIP Respiration Medicine , PLA Navy General Hospital , Beijing , 
      China.
FAU - Li, Yanbo
AU  - Li Y
AD  - c Department of VIP General Medicine , PLA Navy General Hospital , Beijing , 
      China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
FAU - Lv, Yan
AU  - Lv Y
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
FAU - Li, Mingxin
AU  - Li M
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
FAU - Pan, Shuyi
AU  - Pan S
AD  - a Department of Hyperbaric Oxygen , PLA Navy General Hospital , Beijing , China.
LA  - eng
PT  - Journal Article
DEP - 20170721
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide)
RN  - 0 (Carbazoles)
RN  - 0 (Ddit3 protein, rat)
RN  - 0 (GRP78 protein, rat)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Protective Agents)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (Xbp1 protein, rat)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acute Lung Injury/chemically induced/genetics/pathology/*prevention & control
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Carbazoles/pharmacology
MH  - Endoplasmic Reticulum Stress/drug effects/genetics
MH  - Enzyme Activation/drug effects
MH  - Gene Expression Regulation
MH  - Heat-Shock Proteins/genetics/metabolism
MH  - Histone Deacetylase Inhibitors/pharmacology
MH  - Hydrogen/*pharmacology
MH  - Hyperoxia/chemically induced/genetics/pathology/*prevention & control
MH  - Male
MH  - Organ Size/drug effects
MH  - Oxygen/*adverse effects
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Sirtuin 1/antagonists & inhibitors/*genetics/metabolism
MH  - Transcription Factor CHOP/genetics/metabolism
MH  - X-Box Binding Protein 1/genetics/metabolism
OTO - NOTNLM
OT  - Hydrogen; hyperoxic acute lung injury
OT  - Sirtuin 1
OT  - endoplasmic reticulum stress
EDAT- 2017/07/06 06:00
MHDA- 2018/04/18 06:00
CRDT- 2017/07/06 06:00
PHST- 2017/07/06 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
PHST- 2017/07/06 06:00 [entrez]
AID - 10.1080/10715762.2017.1351027 [doi]
PST - ppublish
SO  - Free Radic Res. 2017 Jun;51(6):622-632. doi: 10.1080/10715762.2017.1351027. Epub 
      2017 Jul 21.