PMID- 28676756 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230804 IS - 1663-4365 (Print) IS - 1663-4365 (Electronic) IS - 1663-4365 (Linking) VI - 9 DP - 2017 TI - Inhibition of PirB Activity by TAT-PEP Improves Mouse Motor Ability and Cognitive Behavior. PG - 199 LID - 10.3389/fnagi.2017.00199 [doi] LID - 199 AB - Paired immunoglobulin-like receptor B (PirB), a functional receptor for myelin-associated inhibitory proteins, plays an important role in axon regeneration in injured brains. However, its role in normal brain function with age has not been previously investigated. Therefore in this study, we examined the expression level of PirB in the cerebral cortex, hippocampus and cerebellum of mice at 1 month, 3 months and 18 months of age. The results showed that the expression of PirB increased with age. We further demonstrated that overexpression of PirB inhibited neurite outgrowth in PC12 cells, and this inhibitory activity of PirB could be reversed by TAT-PEP, which is a recombinant soluble PirB ectodomain fused with TAT domain for blood-brain barrier penetration. In vivo study, intraperitoneal administration of TAT-PEP was capable of enhancing motor capacity and spatial learning and memory in mice, which appeared to be mediated through regulation of brain-derived neurotrophic factor (BDNF) secretion. Our study suggests that PirB is associated with aging and TAT-PEP may be a promising therapeutic agent for modulation of age-related motor and cognitive dysfunctions. FAU - Mi, Ya-Jing AU - Mi YJ AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Chen, Hai AU - Chen H AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. AD - Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical UniversityXi'an, China. FAU - Guo, Na AU - Guo N AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Sun, Meng-Yi AU - Sun MY AD - Department of Neurosurgery, School of Medicine, Yale UniversityNew Haven, CT, United States. FAU - Zhao, Zhao-Hua AU - Zhao ZH AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Gao, Xing-Chun AU - Gao XC AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Wang, Xiao-Long AU - Wang XL AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Zhang, Rui-San AU - Zhang RS AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. FAU - Zhou, Jiang-Bing AU - Zhou JB AD - Department of Neurosurgery, School of Medicine, Yale UniversityNew Haven, CT, United States. FAU - Gou, Xing-Chun AU - Gou XC AD - Institute of Basic and Translational Medicine, and School of Basic Medical Sciences, and Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical UniversityXi'an, China. AD - Department of Neurosurgery, School of Medicine, Yale UniversityNew Haven, CT, United States. LA - eng GR - R01 NS095817/NS/NINDS NIH HHS/United States GR - R21 NS095147/NS/NINDS NIH HHS/United States PT - Journal Article DEP - 20170620 PL - Switzerland TA - Front Aging Neurosci JT - Frontiers in aging neuroscience JID - 101525824 PMC - PMC5476690 OTO - NOTNLM OT - BDNF OT - PirB OT - TAT-PEP OT - cognitive behavior OT - motor capacity EDAT- 2017/07/06 06:00 MHDA- 2017/07/06 06:01 PMCR- 2017/01/01 CRDT- 2017/07/06 06:00 PHST- 2017/03/09 00:00 [received] PHST- 2017/06/02 00:00 [accepted] PHST- 2017/07/06 06:00 [entrez] PHST- 2017/07/06 06:00 [pubmed] PHST- 2017/07/06 06:01 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - 10.3389/fnagi.2017.00199 [doi] PST - epublish SO - Front Aging Neurosci. 2017 Jun 20;9:199. doi: 10.3389/fnagi.2017.00199. eCollection 2017.