PMID- 28677360
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 9
IP  - 5
DP  - 2017 Sep
TI  - A Metagenomic Analysis Provides a Culture-Independent Pathogen Detection for 
      Atopic Dermatitis.
PG  - 453-461
LID - 10.4168/aair.2017.9.5.453 [doi]
AB  - PURPOSE: Atopic dermatitis (AD) is an inflammatory skin disease, significantly 
      affecting the quality of life. Using AD as a model system, we tested a successive 
      identification of AD-associated microbes, followed by a culture-independent serum 
      detection of the identified microbe. METHODS: A total of 43 genomic DNA 
      preparations from washing fluid of the cubital fossa of 6 healthy controls, skin 
      lesions of 27 AD patients, 10 of which later received treatment (post-treatment), 
      were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform. 
      RESULTS: Microbial diversity was decreased in AD, and was restored following 
      treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, 
      and Streptococcus, whereas Alcaligenaceae (f), Sediminibacterium, and Lactococcus 
      were characteristic of healthy skin. An enzyme-linked immunosorbent assay (ELISA) 
      showed that serum could be used as a source for the detection of Staphylococcus 
      aureus extracellular vesicles (EVs). S. aureus EV-specific immunoglobulin G (IgG) 
      and immunoglobulin E (IgE) were quantified in the serum. CONCLUSIONS: A 
      metagenomic analysis together with a serum detection of pathogen-specific EVs 
      provides a model for successive identification and diagnosis of pathogens of AD.
CI  - Copyright (c) 2017 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease
FAU - Kim, Min Hye
AU  - Kim MH
AD  - Department of Internal Medicine, Ewha Womans University School of Medicine, 
      Seoul, Korea.
FAU - Rho, Mina
AU  - Rho M
AD  - Department of Computer Science and Engineering, Hanyang University, Seoul, Korea.
FAU - Choi, Jun Pyo
AU  - Choi JP
AD  - Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan 
      Medical Center, Seoul, Korea.
FAU - Choi, Hyun Il
AU  - Choi HI
AD  - Division of Molecular and Life Sciences, Department of Life Science, Pohang 
      University of Science and Technology, Pohang, Korea.
FAU - Park, Han Ki
AU  - Park HK
AD  - Department of Allergy and Clinical Immunology, Ajou University Medical Center, 
      Suwon, Korea.
FAU - Song, Woo Jung
AU  - Song WJ
AD  - Department of Internal Medicine, Seoul National University College of Medicine, 
      Seoul, Korea.
FAU - Min, Taek Ki
AU  - Min TK
AD  - Department of Pediatrics, Pediatric Allergy and Respiratory Center, Soonchunhyang 
      University Hospital, Soonchunhyang University College of Medicine, Seoul, Korea.
FAU - Cho, Sang Heon
AU  - Cho SH
AD  - Department of Internal Medicine, Seoul National University College of Medicine, 
      Seoul, Korea.
FAU - Cho, Young Joo
AU  - Cho YJ
AD  - Department of Internal Medicine, Ewha Womans University School of Medicine, 
      Seoul, Korea.
FAU - Kim, Yoon Keun
AU  - Kim YK
AD  - Institute of MD Healthcare, Seoul, Korea.
FAU - Yang, Sanghwa
AU  - Yang S
AD  - Institute of MD Healthcare, Seoul, Korea. arraychip@gmail.com.
FAU - Pyun, Bok Yang
AU  - Pyun BY
AD  - Department of Pediatrics, Pediatric Allergy and Respiratory Center, Soonchunhyang 
      University Hospital, Soonchunhyang University College of Medicine, Seoul, Korea. 
      bypyun@schmc.ac.kr.
LA  - eng
GR  - 27301C0040/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC5500701
OTO - NOTNLM
OT  - Atopic dermatitis
OT  - extracellular vesicle
OT  - metagenomic analysis
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2017/07/06 06:00
MHDA- 2017/07/06 06:01
PMCR- 2017/09/01
CRDT- 2017/07/06 06:00
PHST- 2016/11/14 00:00 [received]
PHST- 2017/03/27 00:00 [revised]
PHST- 2017/04/04 00:00 [accepted]
PHST- 2017/07/06 06:00 [entrez]
PHST- 2017/07/06 06:00 [pubmed]
PHST- 2017/07/06 06:01 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - 9.453 [pii]
AID - 10.4168/aair.2017.9.5.453 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2017 Sep;9(5):453-461. doi: 
      10.4168/aair.2017.9.5.453.