PMID- 28678563
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20190307
IS  - 1742-8149 (Electronic)
IS  - 1464-7273 (Linking)
VI  - 21
IP  - 4
DP  - 2018 Dec
TI  - Effect of brain-derived neurotrophic factor (BDNF) on sperm quality of 
      normozoospermic men.
PG  - 248-254
LID - 10.1080/14647273.2017.1346301 [doi]
AB  - The neurotrophin family of proteins and their receptors act as important 
      proliferative and pro-survival factors in differentiation of nerve cells and are 
      thought to play key roles in the development of reproductive tissues and normal 
      function of spermatozoa. The objective of the present study was to evaluate the 
      effect of Brain-Derived Neurotrophic Factor (BDNF) on the sperm viability and 
      motility, lipid peroxidation (LPO), mitochondrial activity and concentration of 
      leptin, nitric oxide (NO) and insulin in normozoospermic men. Semen samples from 
      20 normozoospermic men were divided into three groups: (i) control, (ii) BDNF and 
      (iii) BDNF + K252a. BDNF and K252a were added in the dose of 0.133 and 0.1 nM, 
      respectively. Viability was assessed by eosin-nigrosin staining technique, and 
      motility was observed by microscopy. NO concentration and mitochondrial activity 
      were measured with flow cytometry, and LPO was analyzed using enzyme-linked 
      immunosorbent assay (ELISA) kits. Results showed that exogenous BDNF at 0.133 nM 
      could significantly (p < 0.05) influence viability, motility, NO concentration, 
      mitochondrial activity and LPO content. Secretions of insulin and leptin by human 
      sperm were increased in cells exposed to the exogenous BDNF, whereas viability, 
      mitochondrial activity and insulin and leptin secretions were decreased in cells 
      exposed to the K252.
FAU - Safari, Hassan
AU  - Safari H
AD  - a Department of Anatomical sciences , Tehran University of Medical Sciences , 
      Tehran , Iran.
FAU - Khanlarkhani, Neda
AU  - Khanlarkhani N
AD  - b National Institute for Medical Research Development (NIMAD) , Tehran , Iran.
FAU - Sobhani, Aligholi
AU  - Sobhani A
AD  - a Department of Anatomical sciences , Tehran University of Medical Sciences , 
      Tehran , Iran.
FAU - Najafi, Atefeh
AU  - Najafi A
AD  - a Department of Anatomical sciences , Tehran University of Medical Sciences , 
      Tehran , Iran.
FAU - Amidi, Fardin
AU  - Amidi F
AD  - a Department of Anatomical sciences , Tehran University of Medical Sciences , 
      Tehran , Iran.
LA  - eng
PT  - Journal Article
DEP - 20170705
PL  - England
TA  - Hum Fertil (Camb)
JT  - Human fertility (Cambridge, England)
JID - 100888143
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Insulin)
RN  - 0 (Leptin)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Survival/drug effects
MH  - Humans
MH  - Insulin/metabolism
MH  - Leptin/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Mitochondria/drug effects/metabolism
MH  - Nitric Oxide/metabolism
MH  - Semen Analysis
MH  - Sperm Motility/*drug effects
MH  - Spermatozoa/*drug effects/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - human sperm
OT  - lipid peroxidation
OT  - mitochondrial activity
OT  - nerve growth factor
OT  - nitric oxide
EDAT- 2017/07/06 06:00
MHDA- 2019/03/08 06:00
CRDT- 2017/07/06 06:00
PHST- 2017/07/06 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2017/07/06 06:00 [entrez]
AID - 10.1080/14647273.2017.1346301 [doi]
PST - ppublish
SO  - Hum Fertil (Camb). 2018 Dec;21(4):248-254. doi: 10.1080/14647273.2017.1346301. 
      Epub 2017 Jul 5.