PMID- 28678609
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20181113
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 8
IP  - 5
DP  - 2017 Sep 3
TI  - Randomized, controlled trial evaluating the effect of multi-strain probiotic on 
      the mucosal microbiota in canine idiopathic inflammatory bowel disease.
PG  - 451-466
LID - 10.1080/19490976.2017.1334754 [doi]
AB  - The intestinal microbiota is increasingly linked to the pathogenesis of 
      idiopathic inflammatory bowel disease (IBD) in dogs. While studies have reported 
      alterations in fecal (luminal) microbial populations, only limited information is 
      available about the mucosal microbiota of IBD dogs at diagnosis and following 
      medical therapy. Our aim was to characterize the mucosal microbiota and determine 
      the clinical, microbiological, and mucosal homeostatic effects of probiotic 
      treatment in dogs with IBD. Thirty four IBD dogs were randomized to receive 
      standard therapy (ST = diet + prednisone) with or without probiotic. Tissue 
      sections from endoscopic biopsies were evaluated by fluorescence in situ 
      hybridization (FISH) on a quantifiable basis. Disease activity and changes in 
      mucosal microbiota and tight junction protein (TJP) expression were assessed 
      before and after 8 weeks of IBD therapy. ST and ST/probiotic therapy modulated 
      the number of mucosal bacteria of IBD dogs in a similar fashion. Both treatments 
      increased the numbers of total bacteria and individual species residing within 
      adherent mucus, with ST therapy increasing Bifidobacterium spp. and ST/probiotic 
      therapy increasing Lactobacillus spp (P < 0.05 for both), respectively. Both 
      treatments were associated with rapid clinical remission but not improvement in 
      histopathologic inflammation. Probiotic therapy was associated with upregulated 
      (P < 0.05) expression of TJPs E-cadherin, occludin, and zonulin versus ST. The 
      probiotic effect on mucosal bacteria is similar to that of IBD dogs receiving ST. 
      IBD dogs fed probiotic had increased TJP expression suggesting that probiotic may 
      have beneficial effects on mucosal homeostasis.
FAU - White, Robin
AU  - White R
AD  - a Department of Veterinary Clinical Sciences, College of Veterinary Medicine , 
      Iowa State University , Ames , Iowa , USA.
FAU - Atherly, Todd
AU  - Atherly T
AD  - b USDA-ARS , Ames , Iowa , USA.
FAU - Guard, Blake
AU  - Guard B
AD  - c Department of Small Animal Clinical Sciences, Gastrointestinal Laboratory, 
      College of Veterinary Medicine and Biomedical Sciences , Texas A&M University , 
      College Station , TX , USA.
FAU - Rossi, Giacomo
AU  - Rossi G
AD  - d School of Biosciences and Veterinary Medicine , University of Camerino , 
      Camerino , Macerata , Italy.
FAU - Wang, Chong
AU  - Wang C
AD  - e Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine , Iowa State University , Ames , Iowa , USA.
FAU - Mosher, Curtis
AU  - Mosher C
AD  - f Department of Genetics, Development & Cell Biology, College of Liberal Arts and 
      Sciences , Iowa State University , Ames , Iowa , USA.
FAU - Webb, Craig
AU  - Webb C
AD  - g Colorado State University Veterinary Teaching Hospital , Fort Collins , CO , 
      USA.
FAU - Hill, Steve
AU  - Hill S
AUID- ORCID: 0000-0001-6035-7253
AD  - h Veterinary Specialty Hospital - San Diego , CA , USA.
FAU - Ackermann, Mark
AU  - Ackermann M
AD  - i Department of Veterinary Pathology, College of Veterinary Medicine , Iowa State 
      University , Ames , Iowa , USA.
FAU - Sciabarra, Peter
AU  - Sciabarra P
AUID- ORCID: 0000-0002-7569-8701
AD  - a Department of Veterinary Clinical Sciences, College of Veterinary Medicine , 
      Iowa State University , Ames , Iowa , USA.
FAU - Allenspach, Karin
AU  - Allenspach K
AD  - a Department of Veterinary Clinical Sciences, College of Veterinary Medicine , 
      Iowa State University , Ames , Iowa , USA.
FAU - Suchodolski, Jan
AU  - Suchodolski J
AD  - c Department of Small Animal Clinical Sciences, Gastrointestinal Laboratory, 
      College of Veterinary Medicine and Biomedical Sciences , Texas A&M University , 
      College Station , TX , USA.
FAU - Jergens, Albert E
AU  - Jergens AE
AD  - a Department of Veterinary Clinical Sciences, College of Veterinary Medicine , 
      Iowa State University , Ames , Iowa , USA.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20170705
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
SB  - IM
MH  - Animals
MH  - Dog Diseases/*pathology/*therapy
MH  - Dogs
MH  - *Gastrointestinal Microbiome
MH  - Gastrointestinal Tract/pathology
MH  - Histocytochemistry
MH  - Inflammatory Bowel Diseases/pathology/therapy/*veterinary
MH  - Probiotics/*administration & dosage
MH  - Treatment Outcome
PMC - PMC5628651
OTO - NOTNLM
OT  - CIBDAI
OT  - FISH
OT  - IBD
OT  - microbiota
OT  - probiotic
OT  - randomized controlled trial
OT  - tight junction protein
EDAT- 2017/07/06 06:00
MHDA- 2018/05/23 06:00
PMCR- 2018/07/05
CRDT- 2017/07/06 06:00
PHST- 2017/07/06 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/07/06 06:00 [entrez]
PHST- 2018/07/05 00:00 [pmc-release]
AID - 1334754 [pii]
AID - 10.1080/19490976.2017.1334754 [doi]
PST - ppublish
SO  - Gut Microbes. 2017 Sep 3;8(5):451-466. doi: 10.1080/19490976.2017.1334754. Epub 
      2017 Jul 5.