PMID- 28678885
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental 
      tuberculosis infection model in sheep.
PG  - e0180546
LID - 10.1371/journal.pone.0180546 [doi]
LID - e0180546
AB  - BACKGROUND/AIMS: Animal tuberculosis (TB) is a complex animal health problem that 
      causes disruption to trade and significant economic losses. TB involves a 
      multi-host system where sheep, traditionally considered a rare host of this 
      infection, have been recently included. The aims of this study were to develop an 
      experimental TB infection model in sheep with a Mycobacterium caprae field strain 
      isolated from a tuberculous diseased ewe, and to use this to evaluate the safety 
      and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis 
      BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. 
      METHODS: Eighteen 2 month-old lambs were experimentally challenged with M. caprae 
      by the endotracheal route (1.5 x 103 CFU). They were separated per treatment 
      group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), 
      orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n 
      = 6). Clinical, immunological, pathological and bacteriological parameters of 
      infection were measured. RESULTS: All lambs were successfully infected and 
      developed gross TB lesions in the respiratory system. The BCG vaccine conferred 
      considerable protection against experimental TB in lambs, as measured by a 
      reduction of the gross lesion volumes and bacterial load. However, HIMB 
      vaccinated animals did not show protection. CONCLUSIONS: This study proposes a 
      reliable new experimental model for a better understanding of tuberculosis in 
      sheep. BCG vaccination offers an effective prospect for controlling the disease. 
      Moreover alternative doses and/or routes of administration should be considered 
      to evaluate the efficacy of the HIMB vaccine candidate.
FAU - Balseiro, Ana
AU  - Balseiro A
AUID- ORCID: 0000-0002-5121-7264
AD  - SERIDA, Servicio Regional de Investigacion y Desarrollo Agroalimentario, Centro 
      de Biotecnologia Animal, Gijon, Asturias, Spain.
FAU - Altuzarra, Raul
AU  - Altuzarra R
AD  - Departament de Medicina i Cirugia Animals, Universitat Autonoma de Barcelona, 
      Bellaterra, Barcelona, Catalonia, Spain.
FAU - Vidal, Enric
AU  - Vidal E
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Catalonia, Spain.
FAU - Moll, Xavier
AU  - Moll X
AD  - Departament de Medicina i Cirugia Animals, Universitat Autonoma de Barcelona, 
      Bellaterra, Barcelona, Catalonia, Spain.
FAU - Espada, Yvonne
AU  - Espada Y
AD  - Departament de Medicina i Cirugia Animals, Universitat Autonoma de Barcelona, 
      Bellaterra, Barcelona, Catalonia, Spain.
FAU - Sevilla, Iker A
AU  - Sevilla IA
AD  - NEIKER-Tecnalia, Instituto Vasco de Investigacion y Desarrollo Agrario, 
      Departamento de Sanidad Animal, Derio, Bizkaia, Pais Vasco, Spain.
FAU - Domingo, Mariano
AU  - Domingo M
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Catalonia, Spain.
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona, 
      Bellaterra, Barcelona, Catalonia, Spain.
FAU - Garrido, Joseba M
AU  - Garrido JM
AD  - NEIKER-Tecnalia, Instituto Vasco de Investigacion y Desarrollo Agrario, 
      Departamento de Sanidad Animal, Derio, Bizkaia, Pais Vasco, Spain.
FAU - Juste, Ramon A
AU  - Juste RA
AD  - SERIDA, Servicio Regional de Investigacion y Desarrollo Agroalimentario, Centro 
      de Biotecnologia Animal, Gijon, Asturias, Spain.
FAU - Prieto, Miguel
AU  - Prieto M
AD  - SERIDA, Servicio Regional de Investigacion y Desarrollo Agroalimentario, Centro 
      de Biotecnologia Animal, Gijon, Asturias, Spain.
FAU - Perez de Val, Bernat
AU  - Perez de Val B
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Catalonia, Spain.
LA  - eng
PT  - Journal Article
DEP - 20170705
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (BCG Vaccine)
RN  - 0 (Vaccines, Inactivated)
SB  - IM
MH  - Animals
MH  - *BCG Vaccine
MH  - *Mycobacterium bovis/immunology
MH  - Sheep
MH  - Tuberculosis/immunology/*prevention & control/*veterinary
MH  - Vaccination
MH  - Vaccines, Inactivated
PMC - PMC5498051
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/06 06:00
MHDA- 2017/10/03 06:00
PMCR- 2017/07/05
CRDT- 2017/07/06 06:00
PHST- 2017/02/03 00:00 [received]
PHST- 2017/06/16 00:00 [accepted]
PHST- 2017/07/06 06:00 [entrez]
PHST- 2017/07/06 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2017/07/05 00:00 [pmc-release]
AID - PONE-D-17-04423 [pii]
AID - 10.1371/journal.pone.0180546 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 5;12(7):e0180546. doi: 10.1371/journal.pone.0180546. 
      eCollection 2017.