PMID- 28679592
OWN - NLM
STAT- MEDLINE
DCOM- 20171019
LR  - 20200725
IS  - 1522-1466 (Electronic)
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 313
IP  - 4
DP  - 2017 Oct 1
TI  - HIF-1-mediated production of exosomes during hypoxia is protective in renal 
      tubular cells.
PG  - F906-F913
LID - 10.1152/ajprenal.00178.2017 [doi]
AB  - Exosomes are nano-sized vesicles produced and secreted by cells to mediate 
      intercellular communication. The production and function of exosomes in kidney 
      tissues and cells remain largely unclear. Hypoxia is a common pathophysiological 
      condition in kidneys. This study was designed to characterize exosome production 
      during hypoxia of rat renal proximal tubular cells (RPTCs), investigate the 
      regulation by hypoxia-inducible factor-1 (HIF-1), and determine the effect of the 
      exosomes on ATP-depletion-induced tubular cell injury. Hypoxia did not change the 
      average sizes of exosomes secreted by RPTCs, but it significantly increased 
      exosome production in a time-dependent manner. HIF-1 induction with 
      dimethyloxalylglycine also promoted exosome secretion, whereas pharmacological 
      and genetic suppression of HIF-1 abrogated the increase of exosome secretion 
      under hypoxia. The exosomes from hypoxic RPTCs had inhibitory effects on 
      apoptosis of RPTCs following ATP depletion. The protective effects were lost in 
      the exosomes from HIF-1alpha knockdown cells. It is concluded that hypoxia stimulates 
      exosome production and secretion in renal tubular cells. The exosomes from 
      hypoxic cells are protective against renal tubular cell injury. HIF-1 mediates 
      exosome production during hypoxia and contributes to the cytoprotective effect of 
      the exosomes.
CI  - Copyright (c) 2017 the American Physiological Society.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Nephrology, The Third Xiangya Hospital of Central South University, 
      Changsha, China.
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta 
      University, Augusta, Georgia; and.
FAU - Zhou, Xiangjun
AU  - Zhou X
AD  - Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China.
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta 
      University, Augusta, Georgia; and.
FAU - Yao, Qisheng
AU  - Yao Q
AD  - Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Liu, Yutao
AU  - Liu Y
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta 
      University, Augusta, Georgia; and.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Nephrology, The Third Xiangya Hospital of Central South University, 
      Changsha, China.
FAU - Dong, Zheng
AU  - Dong Z
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta 
      University, Augusta, Georgia; and.
AD  - Department of Nephrology, The Second Xiangya Hospital of Central South 
      University, Changsha, China.
LA  - eng
GR  - I01 BX000319/BX/BLRD VA/United States
GR  - R01 DK087843/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20170705
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Amino Acids, Dicarboxylic)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - VVW38EB8YS (oxalylglycine)
SB  - IM
MH  - Amino Acids, Dicarboxylic
MH  - Animals
MH  - Cell Line
MH  - Exosomes/*metabolism
MH  - Hypoxia/*physiopathology
MH  - Hypoxia-Inducible Factor 1/*physiology
MH  - Kidney Tubules, Proximal/*metabolism
MH  - Mice
PMC - PMC5668579
OTO - NOTNLM
OT  - ATP depletion
OT  - HIF-1
OT  - exosome
OT  - hypoxia
OT  - kidney injury
EDAT- 2017/07/07 06:00
MHDA- 2017/10/20 06:00
PMCR- 2018/10/01
CRDT- 2017/07/07 06:00
PHST- 2017/04/04 00:00 [received]
PHST- 2017/06/21 00:00 [revised]
PHST- 2017/06/26 00:00 [accepted]
PHST- 2017/07/07 06:00 [pubmed]
PHST- 2017/10/20 06:00 [medline]
PHST- 2017/07/07 06:00 [entrez]
PHST- 2018/10/01 00:00 [pmc-release]
AID - ajprenal.00178.2017 [pii]
AID - F-00178-2017 [pii]
AID - 10.1152/ajprenal.00178.2017 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2017 Oct 1;313(4):F906-F913. doi: 
      10.1152/ajprenal.00178.2017. Epub 2017 Jul 5.