PMID- 28684695 OWN - NLM STAT- MEDLINE DCOM- 20180330 LR - 20181202 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 9 IP - 7 DP - 2017 Jul 6 TI - Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure. LID - 10.3390/nu9070706 [doi] LID - 706 AB - Dendritic cells' (DCs) ability to present antigens and initiate the adaptive immune response confers them a pivotal role in immunological defense against hostile infection and, at the same time, immunological tolerance towards harmless components of the microbiota. Food products can modulate the inflammatory status of intestinal DCs. Among nutritionally-derived products, we investigated the ability of quercetin to suppress inflammatory cytokines secretion, antigen presentation, and DCs migration towards the draining lymph nodes. We recently identified the Slpi expression as a crucial checkpoint required for the quercetin-induced inflammatory suppression. Here we demonstrate that Slpi-KO DCs secrete a unique panel of cytokines and chemokines following quercetin exposure. In vivo, quercetin-enriched food is able to induce Slpi expression in the ileum, while little effects are detectable in the duodenum. Furthermore, Slpi expressing cells are more frequent at the tip compared to the base of the intestinal villi, suggesting that quercetin exposure could be more efficient for DCs projecting periscopes in the intestinal lumen. These data suggest that quercetin-enriched nutritional regimes may be efficient for suppressing inflammatory syndromes affecting the ileo-colonic tract. FAU - De Santis, Stefania AU - De Santis S AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. stefania.desantis@ispa.cnr.it. AD - Institute of Sciences of Food Production C.N.R., Unit of Lecce, via Monteroni, 73100 Lecce, Italy. stefania.desantis@ispa.cnr.it. FAU - Galleggiante, Vanessa AU - Galleggiante V AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. vanessa.galleggiante@libero.it. FAU - Scandiffio, Letizia AU - Scandiffio L AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. lety.scandiffio@hotmail.it. FAU - Liso, Marina AU - Liso M AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. marinaliso@libero.it. FAU - Sommella, Eduardo AU - Sommella E AD - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. esommella@unisa.it. FAU - Sobolewski, Anastasia AU - Sobolewski A AD - University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK. a.sobolewski@uea.ac.uk. FAU - Spilotro, Vito AU - Spilotro V AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. vito.spilotro@irccsdebellis.it. FAU - Pinto, Aldo AU - Pinto A AD - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. pintoal@unisa.it. FAU - Campiglia, Pietro AU - Campiglia P AD - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. pcampiglia@unisa.it. AD - European Biomedical Research Institute of Salerno (EBRIS), Via S. de Renzi, 3, 84125 Salerno, Italy. pcampiglia@unisa.it. FAU - Serino, Grazia AU - Serino G AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. grazia.serino@irccsdebellis.it. FAU - Santino, Angelo AU - Santino A AD - Institute of Sciences of Food Production C.N.R., Unit of Lecce, via Monteroni, 73100 Lecce, Italy. angelo.santino@ispa.cnr.it. FAU - Notarnicola, Maria AU - Notarnicola M AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. maria.notarnicola@irccsdebellis.it. FAU - Chieppa, Marcello AU - Chieppa M AD - National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. transmed@irccsdebellis.it. AD - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. transmed@irccsdebellis.it. LA - eng PT - Journal Article DEP - 20170706 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Antioxidants) RN - 0 (Chemokine CXCL1) RN - 0 (Cxcl1 protein, mouse) RN - 0 (Secretory Leukocyte Peptidase Inhibitor) RN - 0 (Slpi protein, mouse) RN - 9IKM0I5T1E (Quercetin) SB - IM MH - Animals MH - Antioxidants/pharmacology MH - Bone Marrow Cells/drug effects MH - Cells, Cultured MH - Chemokine CXCL1/genetics/metabolism MH - Dendritic Cells/*drug effects/*metabolism MH - Gene Expression Regulation/drug effects MH - Inflammation/*drug therapy MH - Intestinal Mucosa/metabolism MH - Intestines/drug effects MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Quercetin/*pharmacology MH - Secretory Leukocyte Peptidase Inhibitor/genetics/*metabolism PMC - PMC5537821 OTO - NOTNLM OT - Slpi OT - dendritic cells OT - inflammation OT - quercetin COIS- The authors declare no financial or commercial conflict of interest. EDAT- 2017/07/08 06:00 MHDA- 2018/03/31 06:00 PMCR- 2017/07/01 CRDT- 2017/07/08 06:00 PHST- 2017/04/21 00:00 [received] PHST- 2017/06/29 00:00 [revised] PHST- 2017/07/04 00:00 [accepted] PHST- 2017/07/08 06:00 [entrez] PHST- 2017/07/08 06:00 [pubmed] PHST- 2018/03/31 06:00 [medline] PHST- 2017/07/01 00:00 [pmc-release] AID - nu9070706 [pii] AID - nutrients-09-00706 [pii] AID - 10.3390/nu9070706 [doi] PST - epublish SO - Nutrients. 2017 Jul 6;9(7):706. doi: 10.3390/nu9070706.