PMID- 28685010
OWN - NLM
STAT- MEDLINE
DCOM- 20180131
LR  - 20181113
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2017
DP  - 2017
TI  - Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative 
      Stress in Spinal Cord Ischemia Reperfusion Injury.
PG  - 8640284
LID - 10.1155/2017/8640284 [doi]
LID - 8640284
AB  - Autophagy is upregulated in spinal cord ischemia reperfusion (SCIR) injury; 
      however, its expression mechanism is largely unknown; moreover, whether autophagy 
      plays a neuroprotective or neurodegenerative role in SCIR injury remains 
      controversial. To explore these issues, we created an SCIR injury rat model via 
      aortic arch occlusion. Compared with normal controls, autophagic cell death was 
      upregulated in neurons after SCIR injury. We found that autophagy promoted 
      neuronal cell death during SCIR, shown by a significant number of terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling- (TUNEL-) positive 
      cells colabeled with the autophagy marker microtubule-associated protein 1 light 
      chain 3, while the autophagy inhibitor 3-methyladenine reduced the number of 
      TUNEL-positive cells and restored neurological and motor function. Additionally, 
      we showed that oxidative stress was the main trigger of autophagic neuronal cell 
      death after SCIR injury and N-acetylcysteine inhibited autophagic cell death and 
      restored neurological and motor function in SCIR injury. Finally, we found that 
      hydrogen sulfide (H(2)S) inhibited autophagic cell death significantly by 
      reducing oxidative stress in SCIR injury via the AKT-the mammalian target of 
      rapamycin (mTOR) pathway. These findings reveal that oxidative stress induces 
      autophagic cell death and that H(2)S plays a neuroprotective role by reducing 
      oxidative stress in SCIR.
FAU - Xie, Lei
AU  - Xie L
AUID- ORCID: 0000-0001-5435-7767
AD  - Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School 
      of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 
      197 Ruijin 2nd Road, Shanghai 200025, China.
FAU - Yu, Sifei
AU  - Yu S
AD  - Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School 
      of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 
      197 Ruijin 2nd Road, Shanghai 200025, China.
FAU - Yang, Kai
AU  - Yang K
AD  - Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School 
      of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 
      197 Ruijin 2nd Road, Shanghai 200025, China.
FAU - Li, Changwei
AU  - Li C
AUID- ORCID: 0000-0001-9324-4685
AD  - Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School 
      of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 
      197 Ruijin 2nd Road, Shanghai 200025, China.
FAU - Liang, Yu
AU  - Liang Y
AUID- ORCID: 0000-0002-1927-4875
AD  - Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School 
      of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 
      197 Ruijin 2nd Road, Shanghai 200025, China.
LA  - eng
PT  - Journal Article
DEP - 20170608
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Autophagy/*physiology
MH  - Disease Models, Animal
MH  - Humans
MH  - Hydrogen Sulfide/*metabolism
MH  - Male
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*metabolism
MH  - Spinal Cord Ischemia/*metabolism
PMC - PMC5480044
EDAT- 2017/07/08 06:00
MHDA- 2018/02/01 06:00
PMCR- 2017/06/08
CRDT- 2017/07/08 06:00
PHST- 2016/12/29 00:00 [received]
PHST- 2017/04/01 00:00 [revised]
PHST- 2017/04/23 00:00 [accepted]
PHST- 2017/07/08 06:00 [entrez]
PHST- 2017/07/08 06:00 [pubmed]
PHST- 2018/02/01 06:00 [medline]
PHST- 2017/06/08 00:00 [pmc-release]
AID - 10.1155/2017/8640284 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2017;2017:8640284. doi: 10.1155/2017/8640284. Epub 2017 Jun 
      8.