PMID- 28687414
OWN - NLM
STAT- MEDLINE
DCOM- 20180402
LR  - 20180402
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 212
DP  - 2017 Oct
TI  - Ovarian expression and localization of clathrin (Cltc) components in cutthroat 
      trout, Oncorhynchus clarki: Evidence for Cltc involvement in endocytosis of 
      vitellogenin during oocyte growth.
PG  - 24-34
LID - S1095-6433(17)30142-3 [pii]
LID - 10.1016/j.cbpa.2017.06.021 [doi]
AB  - To evaluate potential involvement of clathrin in endocytosis of vitellogenin 
      (Vtg) by teleost oocytes, cDNAs encoding clathrin heavy chain (cltc) were cloned 
      from ovaries of cutthroat trout. Quantitative PCR revealed three types of cltc 
      (cltc-a1, cltc-a2, cltc-b) to be expressed in 10 different tissues including the 
      ovary. The cltc-a1 alone exhibited a significant decrease in ovarian expression 
      during vitellogenesis; this was correlated with a corresponding decrease in 
      transcripts encoding the major Vtg receptor (Vtgr). No development-related 
      changes in ovarian cltc-a2 or cltc-b transcript levels were observed. In situ 
      hybridization revealed a strong ctlc signal in pre-vitellogenic oocytes, but not 
      in vitellogenic oocytes. Western blotting using a rabbit antiserum (a-Cltc) 
      raised against a recombinant Cltc preparation detected a polypeptide band with an 
      apparent mass of ~170kDa in vitellogenic ovary extracts. Immunohistochemistry 
      using a-Cltc revealed Cltc to be uniformly distributed throughout the ooplasm of 
      perinucleolus stage oocytes, translocated to the periphery of lipid droplet stage 
      oocytes, and localized to the oolemma during vitellogenesis. These patterns of 
      cltc/Cltc distribution and abundance during oogenesis, which are identical to 
      those previously reported for vtgr/Vtgr in this species, constitute the first 
      empirical evidence that cltc-a1/Cltc-a1 is involved in Vtg endocytosis via the 
      Vtgr in teleost fish.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Mizuta, Hiroko
AU  - Mizuta H
AD  - Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan.
FAU - Mushirobira, Yuji
AU  - Mushirobira Y
AD  - Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan.
FAU - Nagata, Jun
AU  - Nagata J
AD  - Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan.
FAU - Todo, Takashi
AU  - Todo T
AD  - Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan.
FAU - Hara, Akihiko
AU  - Hara A
AD  - Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan.
FAU - Reading, Benjamin J
AU  - Reading BJ
AD  - Department of Applied Ecology, North Carolina State University, Raleigh, NC 
      27695-7617, USA.
FAU - Sullivan, Craig V
AU  - Sullivan CV
AD  - Carolina AquaGyn, P. O. Box 12914, Raleigh, NC 27605, USA.
FAU - Hiramatsu, Naoshi
AU  - Hiramatsu N
AD  - Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido 
      University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan. Electronic address: 
      naoshi@fish.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20170704
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Clathrin)
RN  - 0 (Vitellogenins)
SB  - IM
MH  - Animals
MH  - Clathrin/*metabolism
MH  - *Endocytosis
MH  - Female
MH  - Humans
MH  - Oncorhynchus/*metabolism
MH  - Oocytes/*cytology
MH  - Ovary/*metabolism
MH  - Vitellogenins/*metabolism
OTO - NOTNLM
OT  - Clathrin
OT  - Cltc
OT  - Oocyte
OT  - Vitellogenin
OT  - Vitellogenin receptor
OT  - Yolk formation
EDAT- 2017/07/09 06:00
MHDA- 2018/04/03 06:00
CRDT- 2017/07/09 06:00
PHST- 2017/04/14 00:00 [received]
PHST- 2017/06/28 00:00 [revised]
PHST- 2017/06/28 00:00 [accepted]
PHST- 2017/07/09 06:00 [pubmed]
PHST- 2018/04/03 06:00 [medline]
PHST- 2017/07/09 06:00 [entrez]
AID - S1095-6433(17)30142-3 [pii]
AID - 10.1016/j.cbpa.2017.06.021 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2017 Oct;212:24-34. doi: 
      10.1016/j.cbpa.2017.06.021. Epub 2017 Jul 4.