PMID- 28687747
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20231213
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jul 7
TI  - Lamin B1 levels modulate differentiation into neurons during embryonic 
      corticogenesis.
PG  - 4897
LID - 10.1038/s41598-017-05078-6 [doi]
LID - 4897
AB  - Lamin B1, a key component of the nuclear lamina, plays an important role in brain 
      development. Ablation of endogenous Lamin B1 (Lmnb1) in the mouse strongly 
      impairs embryonic brain development and corticogenesis. However, the mechanisms 
      underlying these neurodevelopmental effects are unknown. Here, we report that 
      Lamin B1 levels modulate the differentiation of murine neural stem cells (NSCs) 
      into neurons and astroglial-like cells. In vitro, endogenous Lmnb1 depletion 
      favors NSC differentiation into glial fibrillar acidic protein 
      (GFAP)-immunoreactive cells over neurons, while overexpression of human Lamin B1 
      (LMNB1) increases the proportion of neurons. In Lmnb1-null embryos, neurogenesis 
      is reduced, while in vivo Lmnb1 silencing in mouse embryonic brain by in utero 
      electroporation of a specific Lmnb1 sh-RNA results in aberrant cortical 
      positioning of neurons and increased expression of the astrocytic marker GFAP in 
      the cortex of 7-day old pups. Together, these results indicate that finely tuned 
      levels of Lamin B1 are required for NSC differentiation into neurons, proper 
      expression of the astrocytic marker GFAP and corticogenesis.
FAU - Mahajani, Sameehan
AU  - Mahajani S
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy.
AD  - Universitaetsmedizin Goettingen, Waldweg 33, Goettingen, 37073, Germany.
FAU - Giacomini, Caterina
AU  - Giacomini C
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy.
AD  - Division of Cancer Studies, King's College London, New Hunt's House, Guy's 
      Campus, London, SE1 1UL, UK.
FAU - Marinaro, Federica
AU  - Marinaro F
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy.
FAU - De Pietri Tonelli, Davide
AU  - De Pietri Tonelli D
AUID- ORCID: 0000-0001-9537-8900
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy.
FAU - Contestabile, Andrea
AU  - Contestabile A
AUID- ORCID: 0000-0002-5417-4722
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy.
FAU - Gasparini, Laura
AU  - Gasparini L
AD  - Dept. of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 
      Genova, Italy. laura.gasparini@abbvie.com.
AD  - Abbvie Deutschland GmbH & Co, Knollstr, Ludwigshafen, 67061, Germany. 
      laura.gasparini@abbvie.com.
LA  - eng
GR  - U42 OD012210/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170707
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Lamin Type B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (glial fibrillary astrocytic protein, mouse)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/cytology/*metabolism
MH  - Cell Differentiation
MH  - Cerebral Cortex/cytology/growth & development/*metabolism
MH  - Embryo, Mammalian
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Glial Fibrillary Acidic Protein/*genetics/metabolism
MH  - Lamin Type B/antagonists & inhibitors/*genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Stem Cells/cytology/metabolism
MH  - Neurogenesis/*genetics
MH  - Neurons/cytology/*metabolism
MH  - Pregnancy
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Signal Transduction
PMC - PMC5501862
COIS- The authors declare that they have no competing interests.
EDAT- 2017/07/09 06:00
MHDA- 2019/01/11 06:00
PMCR- 2017/07/07
CRDT- 2017/07/09 06:00
PHST- 2016/12/09 00:00 [received]
PHST- 2017/05/24 00:00 [accepted]
PHST- 2017/07/09 06:00 [entrez]
PHST- 2017/07/09 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
PHST- 2017/07/07 00:00 [pmc-release]
AID - 10.1038/s41598-017-05078-6 [pii]
AID - 5078 [pii]
AID - 10.1038/s41598-017-05078-6 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jul 7;7(1):4897. doi: 10.1038/s41598-017-05078-6.