PMID- 28689650
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20180402
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 35
IP  - 34
DP  - 2017 Aug 3
TI  - Relative contribution of porcine reproductive and respiratory syndrome virus open 
      reading frames 2-4 to the induction of protective immunity.
PG  - 4408-4413
LID - S0264-410X(17)30858-7 [pii]
LID - 10.1016/j.vaccine.2017.06.061 [doi]
AB  - The minor glycoproteins (GPs) of PRRSV, GP2, GP3, and GP4, form a heterotrimer 
      that is required for viral infectivity, presumably due to its interaction with 
      the key cellular receptor CD163. These 3GPs are encoded by open reading frames 
      (ORFs) 2a, 3 and 4 (herein referred to as ORFs 2-4), respectively. The goal of 
      this study was to investigate the immunogenicity of the PRRSV-2 minor GPs. 
      Through the use of reverse genetics, a chimeric virus (designated SDFL24) was 
      constructed by replacing ORFs 2-4 of the PRRSV-1 strain SD01-08 with the 
      corresponding genes of the PRRSV-2 strain FL12. While the parental PRRSV strain 
      SD01-08 was not neutralized by convalescent antisera raised against FL12, the 
      chimeric virus SDFL24 gained susceptibility to neutralization by FL12-specific 
      antisera, indicating that viral proteins encoded by ORFs 2-4 are targets of 
      antibody neutralization. When inoculated into pigs, the chimeric virus SDFL24 
      elicited T-cell responses against peptides derived from FL12 minor GPs, whereas 
      the parental virus SD01-08 did not. After challenge infection with FL12, pigs 
      previously infected with SDFL24 developed robust kinetics of FL12-specific 
      neutralizing antibodies as compared to those previously infected with the 
      parental strain SD01-08. Finally, the pigs recovered from SDFL24 infection were 
      better protected from a subsequent challenge infection with FL12 than those 
      previously infected with SD01-08. Collectively, the results indicate that PRRSV-2 
      ORFs 2-4 are capable of inducing protective immunity.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Kimpston-Burkgren, Kathryn
AU  - Kimpston-Burkgren K
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United 
      States; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, 
      NE, United States.
FAU - Correas, Ignacio
AU  - Correas I
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United 
      States; School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, United States.
FAU - Osorio, Fernando A
AU  - Osorio FA
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United 
      States; School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, United States.
FAU - Steffen, David
AU  - Steffen D
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, United States.
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United 
      States; School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, United States.
FAU - Fang, Ying
AU  - Fang Y
AD  - Departement of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS, United States.
FAU - Vu, Hiep L X
AU  - Vu HLX
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United 
      States; Department of Animal Science, University of Nebraska-Lincoln, Lincoln, 
      NE, United States. Electronic address: hiepvu@unl.edu.
LA  - eng
GR  - P30 GM103509/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170706
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/biosynthesis/blood/immunology
MH  - Antibodies, Viral/blood/immunology
MH  - Immunization
MH  - Membrane Glycoproteins/genetics/*immunology
MH  - *Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Reverse Genetics/methods
MH  - Sus scrofa/immunology
MH  - Swine
MH  - T-Lymphocytes/immunology
MH  - Viral Proteins/immunology
OTO - NOTNLM
OT  - Chimeric virus
OT  - Immunization-challenge experiment
OT  - Minor glycoproteins
OT  - PRRSV
EDAT- 2017/07/12 06:00
MHDA- 2018/03/20 06:00
CRDT- 2017/07/11 06:00
PHST- 2017/04/11 00:00 [received]
PHST- 2017/06/13 00:00 [revised]
PHST- 2017/06/15 00:00 [accepted]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2017/07/11 06:00 [entrez]
AID - S0264-410X(17)30858-7 [pii]
AID - 10.1016/j.vaccine.2017.06.061 [doi]
PST - ppublish
SO  - Vaccine. 2017 Aug 3;35(34):4408-4413. doi: 10.1016/j.vaccine.2017.06.061. Epub 
      2017 Jul 6.