PMID- 28690605
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Dicer-Like Genes Are Required for H(2)O(2) and KCl Stress Responses, 
      Pathogenicity and Small RNA Generation in Valsa mali.
PG  - 1166
LID - 10.3389/fmicb.2017.01166 [doi]
LID - 1166
AB  - Valsa mali (V. mali) is the causative agent of apple tree Valsa canker, which 
      heavily damages the production of apples in China. However, the biological roles 
      of the RNA interfering (RNAi) pathway in the pathogenicity of V. mali remain 
      unknown. Dicer-like proteins (DCLs) are important components that control the 
      initiation of the RNAi pathway. In this study, VmDCL1 and VmDCL2 were isolated 
      and functionally characterized in V. mali. VmDCL1 and VmDCL2 are orthologous in 
      evolution to the DCLs in Cryphonectria parasitica. The deletion of VmDCL1 and 
      VmDCL2 did not affect vegetative growth when the mutants (DeltaVmDCL1, DeltaVmDCL2 and 
      DeltaVmDCL1DCL2) and wild type strain 03-8 were grown on a PDA medium at 25 degrees C in the 
      dark. However, the colony of DeltaVmDCL1 increased by 37.1% compared to the 03-8 
      colony in a medium containing 0.05% H(2)O(2) 3 days after inoculation, and the 
      growth of DeltaVmDCL1 was significantly inhibited in a medium containing 0.5 M KCl at 
      a ratio of 25.7%. Meanwhile, in the presence of 0.05% H(2)O(2), the growth of 
      DeltaVmDCL2 decreased by 34.5% compared with the growth of 03-8, but DeltaVmDCL2 grew 
      normally in the presence of 0.5 M KCl. More importantly, the expression of VmDCL2 
      was up-regulated 125-fold during the pathogen infection. In the infection assays 
      using apple twigs, the pathogenicity of DeltaVmDCL2 and DeltaVmDCL1DCL2 was significantly 
      reduced compared with that of 03-8 at a ratio of 24.7 and 41.3%, respectively. 
      All defective phenotypes could be nearly rescued by re-introducing the wild type 
      VmDCL1 and VmDCL2 alleles. Furthermore, the number and length distribution of 
      unique small RNAs (unisRNAs) in the mutants and 03-8 were analyzed using deep 
      sequencing. The number of unisRNAs was obviously lower in DeltaVmDCL1, DeltaVmDCL2 and 
      DeltaVmDCL1DCL2 than that in 03-8, and the length distribution of the sRNAs also 
      markedly changed after the VmDCLs were deleted. These results indicated that 
      VmDCLs function in the H(2)O(2) and KCl stress response, pathogenicity and 
      generation of sRNAs.
FAU - Feng, Hao
AU  - Feng H
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
FAU - Xu, Ming
AU  - Xu M
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
FAU - Liu, Yangyang
AU  - Liu Y
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
FAU - Dong, Ruqing
AU  - Dong R
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
FAU - Gao, Xiaoning
AU  - Gao X
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
FAU - Huang, Lili
AU  - Huang L
AD  - College of Plant Protection and State Key Laboratory of Crop Stress Biology for 
      Arid Areas, Northwest A&F UniversityYangling, China.
LA  - eng
PT  - Journal Article
DEP - 20170623
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
EIN - Front Microbiol. 2017 Jul 28;8:1457. PMID: 28775723
PMC - PMC5481355
OTO - NOTNLM
OT  - Dicer
OT  - RNAi mechanism
OT  - Valsa mali
OT  - pathogenesis
OT  - small RNA
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/06/23
CRDT- 2017/07/11 06:00
PHST- 2017/03/22 00:00 [received]
PHST- 2017/06/07 00:00 [accepted]
PHST- 2017/07/11 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/06/23 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.01166 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Jun 23;8:1166. doi: 10.3389/fmicb.2017.01166. eCollection 
      2017.